Fitness data for PP_3807 in Pseudomonas putida KT2440

Thioesterase

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.5)

group	condition	fitness	t score
nitrogen source	D-2-Aminobutyric (N)	-0.7	-2.1	compare
pH	Growth at pH6 and (C) D-Glucose	-0.7	-1.7	compare
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs	-0.7	-1.7	compare
agar plate interaction	Parafilmed volatile agar plate with Trichoderma atroviridae IMI	-0.6	-1.8	compare
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs	-0.6	-2.0	compare
stress	MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL	-0.5	-1.8	compare
reactor_pregrowth	Adaptation in M9 media timecourse 3; day 3	+0.5	+3.1	compare
reactor_pregrowth	Adaptation in M9 media timecourse 1; day 3	+0.5	+2.7	compare
reactor	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.	+0.5	+3.0	compare
reactor	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.	+0.5	+2.6	compare
reactor	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.	+0.6	+2.4	compare
phage	P. putida South MOI 0.1	+0.6	+2.9	compare
reactor	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay	+0.6	+2.9	compare
pH	Growth at pH7 and (C) D-Glucose	+0.6	+2.0	compare
reactor	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay	+0.6	+2.9	compare
nitrogen source	Urea (N)	+0.6	+2.4	compare
pH	Growth at pH6 and (C) D-Glucose	+0.6	+1.7	compare
carbon source	ferulate (C); with MOPS	+0.6	+1.8	compare
reactor	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay	+0.7	+3.2	compare
micoeukaryotes	Tetrahymena sp., Mixed culture-2	+1.1	+2.7	compare