Fitness data for PP_3284 in Pseudomonas putida KT2440

enoyl-CoA hydratase-isomerase
SEED: Phenylacetate degradation enoyl-CoA hydratase PaaA (EC 4.2.1.17)
KEGG: enoyl-CoA hydratase

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.9)


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group condition fitness t score  
carbon source Phenylacetic (C) -7.8 -5.4 compare
carbon source Phenylacetic (C) -4.7 -5.3 compare
phage P. putida Sci small MOI 0.1 -2.5 -2.1 compare
phage P. putida Sci small MOI 0.1 -1.7 -1.4 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.2 -1.9 compare
nitrogen source NAG (N); with MOPS -1.1 -1.7 compare
temperature shift Temperature shift 10_to_30 -1.1 -3.0 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.1 -1.6 compare
temperature Growth at 10C; with MOPS -1.0 -3.7 compare
phage moi 100 Time6-phageFRS -1.0 -3.1 compare
stress NaCl stress 250 mM -1.0 -2.0 compare
pH Growth at pH6 and (C) D-Glucose -1.0 -1.4 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.9 -1.5 compare
nitrogen source nitrate (N) -0.9 -1.7 compare
stress R2A with Fusaric 240 ug/mL -0.9 -2.5 compare
r2a control with 0.2% methanol R2A control with 0.2% methanol -0.9 -2.0 compare
pH Growth at pH7 and (C) D-Glucose -0.9 -1.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.9 -2.2 compare
nitrogen source NAG (N); with MOPS +0.9 +1.6 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 +1.8 compare