Fitness data for PP_2557 in Pseudomonas putida KT2440

Sensory box protein
SEED: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.6)


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group condition fitness t score  
phage P. putida Missley MOI 0.1 -1.2 -1.6 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.2 -4.1 compare
nitrogen source D-2-Aminobutyric (N) -0.9 -2.0 compare
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.9 -2.0 compare
phage P. putida Sci small MOI 0.1 -0.8 -1.5 compare
carbon source Crotonic (C) -0.8 -2.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.8 -1.8 compare
temperature shift Temperature shift 30_to_25 -0.8 -2.3 compare
pH Growth at pH9 and (C) Trisodium citrate -0.8 -2.4 compare
stress Growth with Chloride 500 mM; with MOPS -0.7 -2.1 compare
phage P. putida South MOI 10 -0.7 -1.4 compare
stress R2A with Fusaric 480 ug/mL -0.7 -2.4 compare
carbon source 1,3-Butandiol (C) -0.6 -2.4 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -0.6 -2.2 compare
stress Butyrolactam 2 vol% -0.6 -2.3 compare
carbon source L-Lysine (C) -0.6 -1.6 compare
temperature shift Temperature shift 30_to_10; with MOPS +0.6 +2.0 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.6 +1.6 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.8 +3.3 compare
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.8 +2.3 compare