Fitness data for PP_1353 in Pseudomonas putida KT2440

conserved membrane protein of unknown function
SEED: Small-conductance mechanosensitive channel
KEGG: small conductance mechanosensitive channel

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 2.5)


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group condition fitness t score  
stress R2A with Polymyxin B sulfate 0.002 mg/ml -3.8 -3.7 compare
nitrogen source nitrogen source nitrite 1mM -3.2 -4.1 compare
nitrogen source nitrate (N) -3.0 -3.5 compare
nitrogen source nitrogen source nitrate 5mM -2.7 -3.7 compare
carbon source Octanoic (C) -2.6 -1.8 compare
r2a control with 0.2x vogels R2A control with 0.2X Vogels -2.5 -3.0 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +2.5 +1.5 compare
nitrogen source 2,6-diaminopimelic (N) +2.6 +1.3 compare
pH Valerolactone (C) pH 6.5 +2.6 +1.7 compare
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +2.7 +1.8 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. +2.8 +1.7 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +2.8 +1.4 compare
nitrogen source 2,6-diaminopimelic (N) +2.8 +1.4 compare
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. +2.8 +2.3 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +2.9 +2.7 compare
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +3.1 +2.5 compare
carbon source 3-Hydroxydecanoic (C) +3.2 +1.6 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +3.3 +3.4 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +3.6 +2.2 compare
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +3.8 +2.3 compare