Fitness data for PP_1257 in Pseudomonas putida KT2440

putative 1-pyrroline-4-hydroxy-2-carboxylate deaminase
SEED: 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22)
KEGG: dihydrodipicolinate synthase

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.4)


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group condition fitness t score  
carbon source Hydroxy-L-Proline (C) -7.9 -5.5 compare
carbon source Hydroxy-L-Proline (C) -7.2 -8.6 compare
pH pH 6 -0.9 -3.5 compare
phage P. putida South MOI 10 -0.7 -2.8 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 -2.5 compare
carbon source Trisodium citrate (C) -0.6 -1.5 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 -2.9 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 -1.9 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay -0.5 -2.6 compare
temperature shift Temperature shift 10_to_25; with MOPS -0.5 -1.7 compare
phage P. putida Sci small MOI 0.1 -0.5 -1.6 compare
temperature shift Temperature shift 30_to_25; with MOPS -0.5 -2.0 compare
carbon source D-Glucose (C) -0.5 -1.4 compare
nitrogen source nitrogen source nitrite 0.5mM -0.5 -2.1 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 -2.2 compare
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.4 -1.7 compare
nitrogen source 2,6-diaminopimelic (N) +0.4 +1.3 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.5 +2.1 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.5 +2.7 compare
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.6 +2.3 compare