Fitness data for PP_0817 in Pseudomonas putida KT2440

aminotransferase
SEED: Uncharacterized PLP-dependent aminotransferase YfdZ
KEGG: alanine-synthesizing transaminase

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.4)


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group condition fitness t score  
nitrogen source L-Leucine (N) -5.5 -10.3 compare
carbon source ferulate (C); with MOPS -0.7 -2.3 compare
nitrogen source nitrogen source nitrate 5mM -0.5 -2.3 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.5 -1.7 compare
carbon source 1,4-Butanediol (C) -0.5 -2.9 compare
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -2.3 compare
temperature Growth at 30C; with MOPS -0.4 -1.5 compare
stress Growth with Chloride 500 mM; with MOPS -0.4 -1.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 -1.3 compare
dilutelb LB 0.1x with 55.5 mM D-Glucose -0.4 -2.1 compare
carbon source Tween 20 (C) +0.4 +1.5 compare
pH pH 9 +0.4 +1.6 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.4 +2.0 compare
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 +1.8 compare
carbon source carbon source 3-O-methyl-glucose 50 mM +0.4 +1.5 compare
phage P. putida Sci small MOI 0.1 +0.4 +2.0 compare
pH Growth at pH6 and (C) D-Glucose +0.5 +1.4 compare
carbon source carbon source 4-vinylphenol 0.01 mM +0.5 +2.3 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.6 +3.2 compare
pH pH 6 +0.6 +2.7 compare