Fitness data for b4216 in Escherichia coli BW25113

ytfJ: predicted transcriptional regulator (NCBI)
SEED: Protein ytfJ precursor

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.4)

group	condition	fitness	t score
motility	outer cut, LB soft agar motility assay	-0.6	-2.0	compare
control	PBS_cage_1_mouse_2_Cecum_40 hr	-0.4	-1.5	compare
carbon source	carbon source D-Mannose 1mM	-0.4	-1.4	compare
phage	Δdbn_10	-0.4	-1.1	compare
carbon source	carbon source D-Glucose 1mM	-0.4	-1.8	compare
stress	LB Aerobic with Novobiocin 0.0000625 mM	-0.4	-1.9	compare
stress	Nickel (II) chloride 1.5 mM	-0.4	-2.3	compare
stress	LB Anaerobic with Zinc Pyrithione 0.0000025 mM	+0.4	+2.1	compare
predation	6 hr rep2_HD100_10fold_dilution_grewON	+0.4	+1.4	compare
stress	Cholin acetate 150 mM	+0.4	+2.1	compare
stress	methylglyoxal 0.008 vol%	+0.4	+2.3	compare
plasmid transformation	vcDART vector transformation; liquid outgrowth assay	+0.4	+2.1	compare
supernatant	Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x	+0.4	+2.4	compare
phage	P2_phage_0.5625_MOI	+0.4	+2.3	compare
carbon source	carbon source D-Mannose 0.5mM	+0.5	+2.1	compare
fr_100_f2	Fr_100_F2	+0.5	+3.5	compare
phage	T7_phage 0.00015625 MOI TC4	+0.5	+1.9	compare
motility	inner cut, LB soft agar motility assay	+0.6	+2.4	compare
stress	LB Anaerobic with Benzethonium chloride 0.00003125 mM	+0.7	+2.2	compare
attachment_assay_attached_sample	Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB	+0.8	+4.1	compare