Fitness for 1 genes in Pseudomonas putida KT2440

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Group Condition PP_5145
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -5.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -5.8
dilutelb LB 0.1x with 55.5 mM D-Glucose -5.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -4.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -4.9
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -4.1
carbon source 2,3-Butanediol (C) -4.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -4.0
phage PUT8 MOI 0.1 -4.0
micoeukaryotes Acanthamoeba sp., muxed culture-1 -3.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -3.8
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -3.8
nitrogen source D-2-Aminobutyric (N) -3.8
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -3.8
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -3.7
nitrogen source Beta-alanine (N) -3.7
phage PUT8 MOI 0.1 -3.7
phage P. putida PUT10 MOI 1 -3.7
phage P. putida South MOI 1 -3.7
dilutelb LB 0.1x with 55.5 mM D-Glucose -3.6
nitrogen source D-2-Aminobutyric (N) -3.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -3.6
phage P. putida PUT10 MOI 10 -3.6
carbon source 3-methyl-2-oxopentanoic (C) +3.7
nitrogen source L-Methionine (N) +4.0
carbon source 3-methyl-2-oxopentanoic (C) +4.3
carbon source Hexanoic (C) +4.9
carbon source propionate (C) +5.3
carbon source 4-Hydroxyvalerate (C) (40mM) +5.4
carbon source 4-Hydroxyvalerate (C) (40mM) +6.1
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PP_5145