Fitness for 1 genes in Pseudomonas putida KT2440

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Group Condition PP_4606
phage P. putida Sci Big MOI 0.1 -0.6
nitrogen source 2,6-diaminopimelic (N) -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3
phage P. putida Sci small MOI 0.1 -0.3
pH Growth at pH6 and (C) D-Glucose -0.3
stress Growth with Chloride 500 mM -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2
carbon source D-Glucose (C) -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.2
stress MOPS minimal media_Glucose20mM with Lithium chloride 200 mM -0.2
reactor_pregrowth Adaptation in M9 media timecourse 3; day 3 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.3
pH Growth at pH6 and (C) Trisodium citrate +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3
temperature shift Temperature shift 30_to_25; with MOPS +0.3
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL +0.3
nitrogen source nitrogen source nitrite 0.5mM +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3
carbon source ferulate (C); with MOPS +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3
temperature shift Temperature shift 30_to_34; with MOPS +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.3
carbon source ferulate (C); with MOPS +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.5
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PP_4606