Fitness for 1 genes in Pseudomonas putida KT2440

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Group Condition PP_2497
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -3.6
nitrogen source Ammonium chloride (N) -3.5
pH pH 9 -3.0
stress Growth with Chloride 500 mM; with MOPS -2.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.4
phage P. put PUT 10 MOI 10 -1.4
temperature shift Temperature shift 30_to_25 -1.3
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.2
pH Growth at pH8 and (C) D-Glucose -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.2
carbon source L-Leucine (C) -1.2
lb outgrowth control more dilute start LB outgrowth control more dilute start -1.1
pH Growth at pH6 and (C) Trisodium citrate -1.1
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -1.1
nitrogen source nitrogen source nitrate 5mM -1.0
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -1.0
carbon source L-Lysine (C) -1.0
temperature shift Temperature shift 10_to_25 -1.0
nitrogen source D-2-Aminobutyric (N) -1.0
carbon source 5-Aminovaleric 10 mM (C) -1.0
pH pH 8 +1.0
nitrogen source D-2-Aminobutyric (N) +1.1
phage P. putida PUT 7 MOI 1 +1.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 +1.2
phage P. putida Sci Big MOI 0.1 +1.4
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PP_2497