Fitness for 1 genes in Pseudomonas putida KT2440

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Group Condition PP_2409
phage PUT 8 MOI 1 -2.3
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -2.1
pH Valerolactone (C) pH 6.5 -1.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -1.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.7
nitrogen source D-2-Aminobutyric (N) -1.7
temperature Growth at 30C; with MOPS -1.6
phage P. putida Sci Big MOI 0.1 -1.5
nitrogen source 2,6-diaminopimelic (N) -1.5
nitrogen source D-2-Aminobutyric (N) -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.3
pH D-Glucose (C) pH 6 -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2
temperature shift Temperature shift 30_to_21; with MOPS -1.1
toxin pUC18_phdP1_pSU18T_docP1 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.0
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -1.0
stress MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -0.9
nitrogen source NAG (N); with MOPS -0.9
temperature shift Temperature shift 30_to_25 -0.9
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +0.9
temperature shift Temperature shift 10_to_30 +0.9
carbon source D-Glucose (C) 2.5 mM +0.9
phage P. putida South MOI 10 +1.0
carbon source Valerolactone (C) +1.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.2
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PP_2409