Fitness for 1 genes in Pseudomonas putida KT2440

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Group Condition PP_0817
nitrogen source L-Leucine (N) -5.5
carbon source ferulate (C); with MOPS -0.7
nitrogen source nitrogen source nitrate 5mM -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.5
carbon source 1,4-Butanediol (C) -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4
temperature Growth at 30C; with MOPS -0.4
stress Growth with Chloride 500 mM; with MOPS -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4
dilutelb LB 0.1x with 55.5 mM D-Glucose -0.4
carbon source 4-Methyl-2-oxopentanoic (C) -0.4
temperature shift Temperature shift 30_to_21; with MOPS -0.4
nitrogen source Ammonium chloride (N) -0.4
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.4
carbon source 1,5-Pentanediol (C) -0.3
pH Growth at pH6 and (C) D-Glucose -0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3
nitrogen source 2,6-diaminopimelic (N) +0.4
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.4
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.4
carbon source Tween 20 (C) +0.4
pH pH 9 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4
carbon source carbon source 3-O-methyl-glucose 50 mM +0.4
phage P. putida Sci small MOI 0.1 +0.4
pH Growth at pH6 and (C) D-Glucose +0.5
carbon source carbon source 4-vinylphenol 0.01 mM +0.5
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.6
pH pH 6 +0.6
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PP_0817