Fitness for 1 genes in Pseudomonas putida KT2440

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Group Condition PP_0261
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -3.7
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -3.7
carbon source carbon source D-Glucose 55.5 mM -3.5
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -3.4
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -3.3
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -3.3
reactor_pregrowth Adaptation in M9 media timecourse 3; day 3 -3.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -3.2
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM -3.0
carbon source L-Valine (C) -3.0
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -2.6
carbon source M9 minimal media_noCarbon with D-Glucose 10 mM; 30C; then outgrowth in LB -2.6
carbon source carbon source 2-Deoxy-D-glucose 100 mM -2.5
carbon source carbon source Glycerol 108.6 mM -2.4
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB -2.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.3
nitrogen source nitrogen source nitrate 5mM -2.2
reactor_pregrowth Adaptation in M9 media timecourse 1; day 3 -2.2
carbon source carbon source Bisabolene 48.9 mM -2.2
carbon source 4-Hydroxyvalerate (C) (40mM) -2.2
carbon source L-Valine (C) -2.2
carbon source carbon source Bisabolol 45 mM -2.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -2.1
reactor_pregrowth Adaptation in M9 media timecourse 2; day 3 -2.1
carbon source carbon source 4-vinylphenol 0.01 mM -2.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.1
nitrogen source L-Leucine (N) -2.1
carbon source 4-Hydroxyvalerate (C) (40mM) -2.0
nitrogen source DL-2-Aminobutyric (N) +3.4
nitrogen source DL-2-Aminobutyric (N) +3.5
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PP_0261