Fitness for 5 genes in Pseudomonas fluorescens GW456-L13

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500 ntPfGW456L13_858 and PfGW456L13_859 overlap by 4 nucleotidesPfGW456L13_859 and PfGW456L13_860 are separated by 263 nucleotidesPfGW456L13_860 and PfGW456L13_861 are separated by 177 nucleotidesPfGW456L13_861 and PfGW456L13_862 are separated by 60 nucleotides PfGW456L13_858 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 964,293 to 965,456 _858 PfGW456L13_859 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 965,453 to 966,691 _859 PfGW456L13_860 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 966,955 to 967,506 _860 PfGW456L13_861 - Regulator of sigma D, at 967,684 to 968,142 _861 PfGW456L13_862 - Macrophage infectivity potentiator, at 968,203 to 968,868 _862
Group Condition PfGW456L13_858 PfGW456L13_859 PfGW456L13_860 PfGW456L13_861 PfGW456L13_862
nitrogen source L-Proline (N) -2.2 -2.1 +0.1 +0.2 +0.1
stress Dimethyl Sulfoxide 7.5 vol% -1.6 -1.5 -0.2 -0.2 -0.2
carbon source octanoate (C) -1.0 -0.3 -0.3 -0.3 -1.1
control LB control -1.4 -0.4 -0.0 -0.8 -0.1
control LB control -1.1 -0.6 -0.0 -0.6 -0.2
control LB control -1.0 -0.1 -0.1 -0.8 -0.2
stress A22 0.04 mg/ml -1.3 -0.7 +0.0 -0.1 -0.1
lb LB -1.2 -0.3 +0.0 -0.4 -0.2
stress nitrate 200 mM -0.9 -0.8 -0.2 +0.1 -0.2
carbon source L-Malic (C) -0.5 -0.3 -0.6 +0.1 -0.6
nitrogen source L-Aspartic Acid (N) -1.0 -1.0 -0.2 +0.2 +0.1
stress KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x +0.1 -1.0 -0.5 -0.1 -0.1
nitrogen source L-Asparagine (N) -0.9 -0.7 -0.2 +0.1 +0.1
nitrogen source L-Citrulline (N) -0.8 -0.9 -0.2 +0.2 +0.1
stress Paraquat dichloride 0.032 mg/ml -0.9 -0.7 +0.0 +0.1 -0.1
carbon source D-Gluconic Acid (C) -0.8 +0.2 -0.6 -0.2 +0.1
stress Nickel (II) chloride 1 mM -0.9 -0.7 -0.1 +0.3 +0.1
nitrogen source Putrescine (N) -0.6 -0.7 -0.4 +0.4 +0.1
nitrogen source L-Leucine (N) -0.9 -0.4 -0.1 +0.1 +0.3
carbon source D-Mannose (C) -1.2 +0.2 +0.1 +0.3 -0.1
carbon source D-Lactate (C) -0.1 -1.1 +0.3 +0.1 +0.2
carbon source Putrescine (C) -0.5 -0.7 +0.1 +0.4 +0.4
carbon source D,L-Lactate (C) -0.5 +0.2 -0.4 +0.1 +0.5
carbon source D-Fructose (C) +0.7 +0.4 +0.0 +0.2 -0.6
carbon source a-Ketoglutaric (C) +0.9 +0.3 -0.3 +0.5 +0.1
carbon source L-Valine (C) +0.9 +0.5 -0.1 +0.1 -0.0
carbon source L-Tryptophan (C) +1.1 +1.0 -0.7 +0.3 -0.1
carbon source D-Glucosamine Hydrochloride (C) +0.8 +0.5 +0.2 +0.2 -0.1
carbon source L-Tryptophan (C) +1.1 +0.8 +0.1 +0.0 -0.2
carbon source a-Ketoglutaric (C) +1.2 +0.3 +0.5 -0.0 +0.0
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