Fitness for 5 genes in Pseudomonas fluorescens GW456-L13

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500 ntPfGW456L13_1582 and PfGW456L13_1583 are separated by 0 nucleotidesPfGW456L13_1583 and PfGW456L13_1584 overlap by 7 nucleotidesPfGW456L13_1584 and PfGW456L13_1585 overlap by 20 nucleotidesPfGW456L13_1585 and PfGW456L13_1586 are separated by 197 nucleotides PfGW456L13_1582 - Peptide chain release factor 1, at 1,747,034 to 1,748,116 _1582 PfGW456L13_1583 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 1,748,117 to 1,748,947 _1583 PfGW456L13_1584 - Molybdopterin biosynthesis protein MoeB, at 1,748,941 to 1,749,696 _1584 PfGW456L13_1585 - Glutamate racemase (EC 5.1.1.3), at 1,749,677 to 1,750,480 _1585 PfGW456L13_1586 - Lipid A 3-O-deacylase, at 1,750,678 to 1,751,121 _1586
Group Condition PfGW456L13_1582 PfGW456L13_1583 PfGW456L13_1584 PfGW456L13_1585 PfGW456L13_1586
stress KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x N.D. N.D. -3.9 N.D. -0.2
stress KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x N.D. N.D. -3.9 N.D. -0.0
carbon source L-Tryptophan (C) N.D. N.D. -3.7 N.D. +0.0
carbon source L-Histidine (C) N.D. N.D. -2.0 N.D. +0.0
nitrogen source nitrate (N) N.D. N.D. -1.7 N.D. -0.1
carbon source L-Tryptophan (C) N.D. N.D. -1.7 N.D. +0.0
carbon source D-Gluconic Acid (C) N.D. N.D. -1.1 N.D. -0.2
carbon source L-Arabinose (C) N.D. N.D. -1.0 N.D. +0.2
carbon source L-Lactate (C) N.D. N.D. -0.6 N.D. -0.1
stress Vancomycin 0.125 mg/ml N.D. N.D. -0.5 N.D. -0.1
stress Vancomycin 0.5 mg/ml N.D. N.D. -0.5 N.D. -0.1
stress Fusidic 0.08 mg/ml N.D. N.D. -0.3 N.D. -0.2
control LB control N.D. N.D. -0.5 N.D. -0.0
carbon source L-Glutamine (C) N.D. N.D. -0.3 N.D. -0.2
nitrogen source L-Alanine (N) N.D. N.D. -0.3 N.D. -0.1
carbon source L-Malic (C) N.D. N.D. -0.5 N.D. +0.1
stress Paraquat dichloride 0.032 mg/ml N.D. N.D. -0.4 N.D. +0.0
control LB control N.D. N.D. -0.4 N.D. +0.1
stress Chloride 400 mM N.D. N.D. -0.3 N.D. +0.1
carbon source L-Histidine (C) N.D. N.D. -0.3 N.D. +0.2
stress Lomefloxacin 0.0004 mM N.D. N.D. +0.2 N.D. -0.3
carbon source D-Glucose (C) N.D. N.D. +0.3 N.D. -0.3
carbon source D-Fructose (C) N.D. N.D. +0.2 N.D. -0.2
carbon source a-Ketoglutaric (C) N.D. N.D. -0.2 N.D. +0.3
carbon source D-Lactate (C) N.D. N.D. +0.4 N.D. -0.3
stress Dimethyl Sulfoxide 7.5 vol% N.D. N.D. +0.5 N.D. +0.1
carbon source D-Galactose (C) N.D. N.D. +0.4 N.D. +0.1
carbon source D-Mannose (C) N.D. N.D. +0.4 N.D. +0.2
carbon source L-Alanine (C) N.D. N.D. +0.5 N.D. +0.1
carbon source a-Ketoglutaric (C) N.D. N.D. +0.9 N.D. -0.0
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