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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_t32 and PP_t33 are separated by 99 nucleotides
PP_t33 and PP_t34 are separated by 141 nucleotides
PP_t34 and PP_2003 are separated by 83 nucleotides
PP_2003 and PP_2004 are separated by 290 nucleotides
PP_t32: PP_t32 - tRNA-Asp, at 2,271,540 to 2,271,616
_t32
PP_t33: PP_t33 - tRNA-Asp, at 2,271,716 to 2,271,792
_t33
PP_t34: PP_t34 - tRNA-Asp, at 2,271,934 to 2,272,010
_t34
PP_2003: PP_2003 - conserved protein of unknown function, at 2,272,094 to 2,273,035
_2003
PP_2004: PP_2004 - Transcriptional regulator, AraC family, at 2,273,326 to 2,274,366
_2004
Group
Condition
PP
_t32
PP
_t33
PP
_t34
PP
_2003
PP
_2004
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
-1.3
-0.4
nitrogen source
Propandiamine (N)
N.D.
N.D.
N.D.
-1.4
-0.1
phage
P. putida Sci small MOI 0.1
N.D.
N.D.
N.D.
-0.7
-0.7
phage
PUT 8 MOI 1
N.D.
N.D.
N.D.
-1.0
-0.3
phage
P. putida Sci Big MOI 0.1
N.D.
N.D.
N.D.
-1.0
-0.2
stress
Geraniol 1 vol%
N.D.
N.D.
N.D.
-1.1
-0.0
nitrogen source
NAG (N); with MOPS
N.D.
N.D.
N.D.
-0.8
-0.4
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM
N.D.
N.D.
N.D.
-1.3
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
N.D.
N.D.
-1.0
-0.1
carbon source
Lignin Monomers (C)
N.D.
N.D.
N.D.
-0.8
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
-0.8
-0.2
carbon source
butyrate (C)
N.D.
N.D.
N.D.
-0.7
-0.3
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
N.D.
N.D.
N.D.
-0.9
-0.1
phage
P. putida PUT 7 MOI 1
N.D.
N.D.
N.D.
-0.8
-0.2
carbon source
butyrate (C)
N.D.
N.D.
N.D.
-0.8
-0.1
carbon source
1,3-Butandiol (C)
N.D.
N.D.
N.D.
-0.6
-0.3
stress
MOPS minimal media_Glucose20mM with Chloride 200 mM
N.D.
N.D.
N.D.
-0.9
+0.1
pH
Growth at pH9 and (C) Trisodium citrate
N.D.
N.D.
N.D.
-1.0
+0.2
carbon source
5-Aminovaleric 10 mM (C)
N.D.
N.D.
N.D.
+0.7
-0.3
carbon source
carbon source 3-O-methyl-glucose 50 mM
N.D.
N.D.
N.D.
+0.7
-0.3
nitrogen source
Urea (N)
N.D.
N.D.
N.D.
+0.8
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
N.D.
N.D.
+0.9
-0.2
carbon source
D-Glucose (C)
N.D.
N.D.
N.D.
+1.0
-0.2
no stress control
Growth with no stress
N.D.
N.D.
N.D.
+1.0
-0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
N.D.
N.D.
+1.0
-0.1
no stress control
Growth with no stress
N.D.
N.D.
N.D.
+0.9
+0.1
pH
pH 9
N.D.
N.D.
N.D.
+0.6
+0.4
carbon source
carbon source 4-vinylphenol 0.1 mM
N.D.
N.D.
N.D.
+0.9
+0.1
temperature
Growth at 30C
N.D.
N.D.
N.D.
+1.0
+0.1
pH
pH 7
N.D.
N.D.
N.D.
+1.0
+0.2
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