Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_t09 and PP_t10 are separated by 46 nucleotidesPP_t10 and PP_t11 are separated by 5 nucleotidesPP_t11 and PP_0626 are separated by 190 nucleotidesPP_0626 and PP_0627 are separated by 84 nucleotides PP_t09: PP_t09 - tRNA-Lys, at 733,551 to 733,626 _t09 PP_t10: PP_t10 - tRNA-Pro, at 733,673 to 733,749 _t10 PP_t11: PP_t11 - tRNA-Lys, at 733,755 to 733,830 _t11 PP_0626: PP_0626 - NADH dehydrogenase, at 734,021 to 735,316 _0626 PP_0627: PP_0627 - conserved protein of unknown function, at 735,401 to 735,574 _0627
Group Condition PP_t09 PP_t10 PP_t11 PP_0626 PP_0627
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -4.6 -1.0 -0.3 -4.2
stress Growth with Chloride 200 mM N.D. -4.3 -3.2 -0.1 +0.8
pH Growth at pH7 and (C) D-Glucose N.D. -4.3 -0.7 -0.6 +0.3
phage P. putida PUT 7 MOI 1 N.D. -3.5 -1.2 -0.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.7 -2.1 +0.4 -0.3
temperature shift Temperature shift 10_to_25; with MOPS N.D. -1.5 -2.7 +0.0 +0.1
pH Valerolactone (C) pH 6.5 N.D. -1.4 -0.8 -0.1 -1.6
pH pH 4 N.D. -1.2 +1.3 -0.6 -3.5
pH Growth at pH6 and (C) Trisodium citrate N.D. -2.9 -0.6 -0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. -2.0 -2.2 +0.4 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -4.5 +0.3 +0.1 +0.5
carbon source carbon source D-Glucose 55.5 mM N.D. -3.7 +3.3 -1.2 -1.7
carbon source carbon source Bisabolene 48.9 mM N.D. -2.4 +1.3 -0.5 -1.1
pH Growth at pH7 and (C) Trisodium citrate N.D. -0.7 -2.7 +0.2 +1.0
stress Growth with Chloride 500 mM; with MOPS N.D. -1.4 -1.5 -0.1 +1.0
pH pH 8 N.D. -2.6 +1.8 -0.4 -0.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -2.6 -0.3 +0.5 +0.5
pH pH 7 N.D. -1.9 +1.3 -0.4 -0.5
nitrogen source nitrogen source nitrate 5mM N.D. -3.0 +0.2 +0.4 +0.9
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. -2.3 +0.4 -0.5 +1.5
temperature Growth at 25C N.D. +1.1 -2.2 +0.3 +0.2
carbon source carbon source 2-Deoxy-D-glucose 50 mM N.D. -1.4 +1.9 -0.3 -0.6
pH pH 5 N.D. -0.2 +1.9 -0.2 -1.5
carbon source carbon source 4-vinylphenol 0.1 mM N.D. -1.6 +2.7 -0.4 -0.5
pH pH 6 N.D. -1.7 +2.7 -0.5 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.8 +1.7 +0.5 -0.9
carbon source carbon source Bisabolol 45 mM N.D. -1.1 +2.5 -0.5 -0.1
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. -1.0 +2.8 -0.1 -0.0
carbon source carbon source 4-vinylphenol 0.01 mM N.D. -0.3 +3.1 -0.0 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +3.0 -0.9 +0.1 +1.0
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