Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_t06 and PP_23SD are separated by 243 nucleotidesPP_23SD and PP_5SD are separated by 131 nucleotidesPP_5SD and PP_0598 are separated by 258 nucleotidesPP_0598 and PP_5745 overlap by 14 nucleotides PP_t06: PP_t06 - tRNA-Ala, at 699,532 to 699,607 _t06 PP_23SD: PP_23SD - 23S ribosomal RNA, at 699,851 to 702,752 _23SD PP_5SD: PP_5SD - 5S ribosomal RNA, at 702,884 to 703,003 _5SD PP_0598: PP_0598 - paraquat-inducible protein A, at 703,262 to 703,921 _0598 PP_5745: PP_5745 - paraquat-inducible protein A, at 703,908 to 704,531 _5745
Group Condition PP_t06 PP_23SD PP_5SD PP_0598 PP_5745
carbon source 2,3-Butanediol (C) N.D. N.D. N.D. -4.5 -3.6
dilutelb LB 0.1x with 55.5 mM D-Glucose N.D. N.D. N.D. -2.0 -2.3
dilutelb LB 0.1x with 55.5 mM D-Glucose N.D. N.D. N.D. -1.1 -1.1
phage PUT8 MOI 10 N.D. N.D. N.D. -1.3 -0.9
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. N.D. N.D. -0.8 -1.1
stress Geraniol 1 vol% N.D. N.D. N.D. -0.9 -0.9
micoeukaryotes Acanthamoeba sp., muxed culture-1 N.D. N.D. N.D. -0.8 -0.9
stress 2-Ethylhexanol 0.4 vol% N.D. N.D. N.D. -1.0 -0.8
phage PUT8 MOI 10 N.D. N.D. N.D. -0.6 -1.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. N.D. N.D. -0.9 -0.8
carbon source 3-methyl-1-butanol 0.1 vol% (C) N.D. N.D. N.D. -0.9 -0.7
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. N.D. N.D. -0.9 -0.6
phage PUT8 MOI 0.1 N.D. N.D. N.D. -1.0 -0.5
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. N.D. N.D. -0.7 -0.9
supernatant LB 0.1x with 55.5 mM D-Glucose with Supernatant: Acinetobacter N.D. N.D. N.D. -0.9 -0.7
stress R2A with Fusaric 480 ug/mL N.D. N.D. N.D. -0.6 -0.8
phage PUT8 MOI 0.1 N.D. N.D. N.D. -1.0 -0.4
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. N.D. N.D. -0.7 -0.7
phage PUT 8 MOI 1 N.D. N.D. N.D. -0.7 -0.6
phage P. putida PUT10 MOI 10 N.D. N.D. N.D. -0.8 -0.6
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. N.D. N.D. -0.6 -0.6
phage P. putida South MOI 1 N.D. N.D. N.D. -0.6 -0.5
micoeukaryotes Dictyostellum sp.mixed culture-1 N.D. N.D. N.D. -0.6 -0.5
stress 1-Pentanol 0.3 vol% N.D. N.D. N.D. +0.3 +0.8
carbon source 3-Hydroxydecanoic (C) N.D. N.D. N.D. +0.6 +0.6
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. N.D. N.D. N.D. +0.5 +0.8
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. N.D. N.D. +0.7 +0.8
carbon source Delta-Decalactone (C) N.D. N.D. N.D. +1.2 +1.0
carbon source Delta-Decalactone (C) N.D. N.D. N.D. +1.6 +1.5
carbon source Delta-Nonalactone (C) N.D. N.D. N.D. +1.8 +1.8
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