Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5465 and PP_1117 are separated by 562 nucleotidesPP_1117 and PP_5742 are separated by 606 nucleotidesPP_5742 and PP_1118 are separated by 42 nucleotidesPP_1118 and PP_1119 are separated by 18 nucleotides PP_5465: PP_5465 - conserved protein of unknown function, at 1,277,964 to 1,278,173 _5465 PP_1117: PP_1117 - conserved protein of unknown function, at 1,278,736 to 1,279,995 _1117 PP_5742: PP_5742 - putative membrane protein of unknown function, at 1,280,602 to 1,281,174 _5742 PP_1118: PP_1118 - Recombinase-related protein, at 1,281,217 to 1,282,908 _1118 PP_1119: PP_1119 - conserved protein of unknown function, at 1,282,927 to 1,283,409 _1119
Group Condition PP_5465 PP_1117 PP_5742 PP_1118 PP_1119
carbon source D-Glucose (C) -0.2 +0.1 -0.8 -0.2 -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay -1.1 +0.4 -0.2 -0.0 -0.3
pH Valerolactone (C) pH 6.5 -0.7 +0.3 -0.6 -0.0 +0.1
nitrogen source Propiolactam (N) -1.0 +0.5 -0.1 +0.0 -0.0
nitrogen source Propiolactam (N) -0.9 +0.7 +0.1 +0.0 -0.3
nitrogen source Hydantoin (N) -0.8 +0.3 +0.3 +0.1 -0.1
nitrogen source L-Phenylalanine (N) -1.3 +0.2 +0.3 +0.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 -0.1 +0.7 +0.2 -0.4
nitrogen source 6-aminocaproic (N) -0.9 +0.3 +0.1 +0.1 +0.2
temperature shift Temperature shift 30_to_21 -0.4 +0.6 +0.3 -0.0 -0.4
carbon source ferulate (C); with MOPS -0.8 -0.0 +0.7 -0.1 +0.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay -0.7 +0.9 +0.2 -0.0 -0.3
carbon source 3-Hydroxydecanoic (C) -0.7 +0.5 -0.0 -0.1 +0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.2 +0.7 -0.1 -0.5
nitrogen source 2,6-diaminopimelic (N) -1.1 +0.8 +0.0 +0.1 +0.5
nitrogen source nitrate (N) -0.7 +0.2 +0.7 +0.0 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.3 +0.2 -0.7 +0.1 +0.5
nitrogen source L-Methionine (N) -0.6 +0.4 +0.2 +0.1 +0.4
carbon source 1,3-Hexanediol (C) -0.5 +0.3 +0.3 +0.0 +0.6
nitrogen source L-Methionine (N) -0.4 +0.5 +0.0 +0.1 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.5 +0.6 +0.4 +0.0 +0.6
carbon source 2-Keto-D-gluconic hemicalcium salt (C) +0.2 +0.3 +0.4 +0.2 +0.5
phage PUT8 MOI 10 +0.5 +0.4 +0.3 +0.1 +0.4
phage P. putida compost MOI 0.1 +0.5 +0.3 +0.5 +0.2 +0.3
phage PUT8 MOI 10 +0.8 +0.1 +0.7 -0.0 +0.3
phage P. putida Sci Big MOI 0.1 +1.0 +0.9 +0.1 +0.3 -0.5
phage P. putida PUT 7 MOI 1 +1.1 +0.6 +0.9 +0.6 +0.0
phage P. putida Missley MOI 0.1 +1.2 +0.9 +0.7 +0.6 +0.6
phage P. putida Sci small MOI 0.1 +1.1 +0.8 +1.3 +0.3 +0.6
phage P. putida Sci small MOI 0.1 +0.9 +0.9 +1.3 +0.6 +0.7
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