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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5402 and PP_5740 are separated by 207 nucleotides
PP_5740 and PP_5741 are separated by 58 nucleotides
PP_5741 and PP_5403 are separated by 54 nucleotides
PP_5403 and PP_5404 are separated by 22 nucleotides
PP_5402: PP_5402 - deacetylase of acetyl-CoA synthetase, NAD-dependent, at 6,159,531 to 6,160,319
_5402
PP_5740: PP_5740 - protein of unknown function, at 6,160,527 to 6,161,183
_5740
PP_5741: PP_5741 - conserved protein of unknown function, at 6,161,242 to 6,161,538
_5741
PP_5403: PP_5403 - conserved protein of unknown function, at 6,161,593 to 6,161,904
_5403
PP_5404: PP_5404 - Transposition protein, TnsC-related protein, at 6,161,927 to 6,163,273
_5404
Group
Condition
PP
_5402
PP
_5740
PP
_5741
PP
_5403
PP
_5404
carbon source
Butyl stearate (C)
-0.3
-0.2
-0.3
-0.4
-0.2
carbon source
Octanoic (C)
-0.3
-0.2
-0.3
-0.4
-0.2
phage
P. putida PUT10 MOI 0.1
-0.3
-0.2
-0.4
-0.3
-0.1
carbon source
Octanoic (C)
-0.2
-0.3
-0.2
-0.4
-0.1
carbon source
Nonanoic (C)
-0.4
-0.3
-0.1
-0.3
-0.1
carbon source
Heptanoic (C)
-0.3
-0.2
-0.1
-0.5
-0.2
no stress control
Growth with no stress
-0.1
+0.0
-0.4
-0.7
+0.1
carbon source
carbon source 3-O-methyl-glucose 50 mM
-0.5
-0.3
-0.2
+0.2
-0.0
r2a control with 0.2% methanol
R2A control with 0.2% methanol
-0.3
-0.1
+0.3
-0.2
-0.2
carbon source
D-Glucose (C)
+0.4
-0.3
-0.0
-0.5
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
+0.2
+0.3
-0.4
-0.2
pH
Growth at pH6 and (C) D-Glucose
-0.0
+0.4
-0.5
+0.2
-0.2
nitrogen source
nitrogen source nitrite 0.5mM
+0.2
-0.2
+0.4
-0.3
-0.2
carbon source
ferulate (C); with MOPS
-0.2
+0.0
+0.4
-0.6
+0.3
nitrogen source
NAG (N); with MOPS
+0.2
-0.0
-0.3
+0.4
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.1
+0.3
-0.3
+0.5
+0.0
nitrogen source
2,6-diaminopimelic (N)
+0.4
+0.0
-0.3
+0.3
+0.2
phage
P. putida Sci Big MOI 0.1
+0.3
+0.1
-0.2
+0.3
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.2
+0.4
-0.3
+0.3
phage
P. putida South MOI 10
+0.3
+0.1
+0.4
+0.4
-0.0
phage
P. putida Sci small MOI 0.1
+0.1
+0.0
+0.2
+0.5
+0.3
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.0
+0.3
+0.2
+0.5
+0.1
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
+0.1
+0.0
+0.7
-0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.3
+0.1
+0.4
+0.3
+0.1
phage
P. put South MOI 10
+0.3
+0.3
+0.3
+0.3
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.2
+0.4
+0.6
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.0
+0.2
+0.4
+0.7
+0.3
phage
P. putida Sci small MOI 0.1
+0.4
+0.5
+0.5
+0.4
+0.5
phage
P. putida Missley MOI 0.1
+0.7
+0.7
+0.4
+0.6
+0.5
phage
P. putida PUT 7 MOI 1
+0.6
+0.5
+0.7
+0.7
+0.6
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