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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4084 and PP_4085 are separated by 53 nucleotides
PP_4085 and PP_5652 are separated by 23 nucleotides
PP_5652 and PP_5654 are separated by 2259 nucleotides
PP_5654 and PP_4091 are separated by 4563 nucleotides
PP_4084: PP_4084 - conserved protein of unknown function, at 4,613,145 to 4,613,576
_4084
PP_4085: PP_4085 - putative RHS family protein, at 4,613,630 to 4,618,222
_4085
PP_5652: PP_5652 - conserved protein of unknown function, phage origin, at 4,618,246 to 4,618,731
_5652
PP_5654: PP_5654 - putative phosphotriesterase, at 4,620,991 to 4,621,407
_5654
PP_4091: PP_4091 - transposase, at 4,625,971 to 4,627,503
_4091
Group
Condition
PP
_4084
PP
_4085
PP
_5652
PP
_5654
PP
_4091
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-1.2
-0.1
-5.1
-2.0
N.D.
carbon source
D-Glucose (C)
-0.1
-0.4
-6.6
-0.7
N.D.
nitrogen source
no extra (N); with MOPS
+0.2
-0.1
-3.5
-1.4
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.4
+0.1
-2.7
-0.9
N.D.
carbon source
Delta-Nonalactone (C)
-0.2
-0.0
-1.2
-2.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.0
-3.2
+0.4
N.D.
nitrogen source
Propandiamine (N)
+0.2
-0.2
-3.0
-0.3
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.6
+0.1
-4.0
+0.4
N.D.
carbon source
ferulate (C); with MOPS
-0.5
+0.1
-2.3
-0.2
N.D.
nitrogen source
nitrogen source nitrate 5mM
+0.4
+0.1
-1.4
-1.9
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.4
+0.0
-1.1
-1.4
N.D.
carbon source
carbon source Bisabolol 45 mM
-0.7
-0.2
-1.4
-0.5
N.D.
carbon source
carbon source D-Glucose 55.5 mM
-0.5
-0.2
-1.8
-0.4
N.D.
pH
pH 5
-0.6
+0.1
-2.2
-0.1
N.D.
carbon source
Trisodium citrate 10 mM (C)
+0.1
-0.1
-2.7
-0.2
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
+0.3
+0.1
-4.0
+0.9
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+0.1
-2.9
+0.3
N.D.
nitrogen source
D-2-Aminobutyric (N)
+0.2
-0.1
-1.4
-1.3
N.D.
nitrogen source
Propiolactam (N)
+0.3
-0.1
-1.0
-1.9
N.D.
nitrogen source
2,6-diaminopimelic (N)
+0.4
+0.2
-2.8
+0.0
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.6
-0.0
-2.2
-0.4
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-0.2
-1.3
-0.8
N.D.
carbon source
L-Leucine (C)
+0.6
+0.0
-1.4
-0.9
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-0.7
+0.2
+0.9
-1.4
N.D.
carbon source
Trisodium citrate (C)
+0.4
+0.1
-1.9
+0.7
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.5
-0.1
-1.5
+0.7
N.D.
pH
Valerolactone (C) pH 6.5
-0.5
-0.1
+1.8
-0.6
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.3
+0.1
-0.6
+0.7
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.1
-0.2
+0.7
+1.9
N.D.
phage
P. putida Missley MOI 0.1
-0.1
+0.3
+1.5
+1.2
N.D.
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