Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4084 and PP_4085 are separated by 53 nucleotidesPP_4085 and PP_5652 are separated by 23 nucleotidesPP_5652 and PP_5654 are separated by 2259 nucleotidesPP_5654 and PP_4091 are separated by 4563 nucleotides PP_4084: PP_4084 - conserved protein of unknown function, at 4,613,145 to 4,613,576 _4084 PP_4085: PP_4085 - putative RHS family protein, at 4,613,630 to 4,618,222 _4085 PP_5652: PP_5652 - conserved protein of unknown function, phage origin, at 4,618,246 to 4,618,731 _5652 PP_5654: PP_5654 - putative phosphotriesterase, at 4,620,991 to 4,621,407 _5654 PP_4091: PP_4091 - transposase, at 4,625,971 to 4,627,503 _4091
Group Condition PP_4084 PP_4085 PP_5652 PP_5654 PP_4091
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.2 -0.1 -5.1 -2.0 N.D.
carbon source D-Glucose (C) -0.1 -0.4 -6.6 -0.7 N.D.
nitrogen source no extra (N); with MOPS +0.2 -0.1 -3.5 -1.4 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.4 +0.1 -2.7 -0.9 N.D.
carbon source Delta-Nonalactone (C) -0.2 -0.0 -1.2 -2.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 -0.0 -3.2 +0.4 N.D.
nitrogen source Propandiamine (N) +0.2 -0.2 -3.0 -0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.6 +0.1 -4.0 +0.4 N.D.
carbon source ferulate (C); with MOPS -0.5 +0.1 -2.3 -0.2 N.D.
nitrogen source nitrogen source nitrate 5mM +0.4 +0.1 -1.4 -1.9 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 +0.0 -1.1 -1.4 N.D.
carbon source carbon source Bisabolol 45 mM -0.7 -0.2 -1.4 -0.5 N.D.
carbon source carbon source D-Glucose 55.5 mM -0.5 -0.2 -1.8 -0.4 N.D.
pH pH 5 -0.6 +0.1 -2.2 -0.1 N.D.
carbon source Trisodium citrate 10 mM (C) +0.1 -0.1 -2.7 -0.2 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.3 +0.1 -4.0 +0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.1 -2.9 +0.3 N.D.
nitrogen source D-2-Aminobutyric (N) +0.2 -0.1 -1.4 -1.3 N.D.
nitrogen source Propiolactam (N) +0.3 -0.1 -1.0 -1.9 N.D.
nitrogen source 2,6-diaminopimelic (N) +0.4 +0.2 -2.8 +0.0 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.6 -0.0 -2.2 -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.2 -1.3 -0.8 N.D.
carbon source L-Leucine (C) +0.6 +0.0 -1.4 -0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.7 +0.2 +0.9 -1.4 N.D.
carbon source Trisodium citrate (C) +0.4 +0.1 -1.9 +0.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.5 -0.1 -1.5 +0.7 N.D.
pH Valerolactone (C) pH 6.5 -0.5 -0.1 +1.8 -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 +0.1 -0.6 +0.7 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 -0.2 +0.7 +1.9 N.D.
phage P. putida Missley MOI 0.1 -0.1 +0.3 +1.5 +1.2 N.D.
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