Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5571 and PP_5572 are separated by 8 nucleotidesPP_5572 and PP_5573 are separated by 703 nucleotidesPP_5573 and PP_3102 are separated by 89 nucleotidesPP_3102 and PP_3103 overlap by 4 nucleotides PP_5571: PP_5571 - conserved protein of unknown function, at 3,502,704 to 3,503,015 _5571 PP_5572: PP_5572 - protein of unknown function, at 3,503,024 to 3,503,305 _5572 PP_5573: PP_5573 - protein of unknown function, at 3,504,009 to 3,504,185 _5573 PP_3102: PP_3102 - conserved protein of unknown function, at 3,504,275 to 3,504,571 _3102 PP_3103: PP_3103 - conserved protein of unknown function, at 3,504,568 to 3,505,641 _3103
Group Condition PP_5571 PP_5572 PP_5573 PP_3102 PP_3103
pH Growth at pH7 and (C) Trisodium citrate -5.6 +0.3 N.D. -1.9 +0.4
carbon source Myristic (C) -5.2 +0.6 N.D. -2.0 +0.0
carbon source ferulate (C); with MOPS -3.2 -0.7 N.D. -1.8 +0.4
phage P. put South MOI 10 -6.2 +0.8 N.D. +0.1 +0.1
carbon source Octanoic (C) -3.7 +0.2 N.D. -1.5 +0.0
temperature shift Temperature shift 10_to_25; with MOPS -2.9 -1.9 N.D. -0.0 +0.1
nitrogen source Ammonium chloride (N) -2.5 -0.8 N.D. -0.6 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -3.1 -0.5 N.D. -0.5 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.9 -1.5 N.D. -0.0 -0.2
temperature shift Temperature shift 10_to_30; with MOPS -3.2 +0.1 N.D. -0.3 +0.0
temperature shift Temperature shift 10_to_30; with MOPS -3.3 +0.6 N.D. -0.9 +0.2
pH Growth at pH8 and (C) D-Glucose -0.8 -0.8 N.D. -1.9 +0.2
nitrogen source nitrogen source nitrite 0.5mM -2.2 +0.4 N.D. -0.8 -0.3
nitrogen source no extra (N); with MOPS -1.6 -0.5 N.D. -1.0 +0.3
pH pH 9 -2.0 +0.2 N.D. -1.1 +0.1
phage P. putida PUT 7 MOI 1 -3.7 -0.6 N.D. +1.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -2.2 -0.7 N.D. +0.2 +0.2
pH Growth at pH9 and (C) Trisodium citrate -2.9 +0.5 N.D. -0.4 +0.4
carbon source carbon source 2-Deoxy-D-glucose 10 mM -1.4 +0.6 N.D. -1.5 +0.1
carbon source carbon source Bisabolene 48.9 mM -1.1 +0.8 N.D. -1.6 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.2 +0.8 N.D. -1.3 -0.1
nitrogen source D-2-Aminobutyric (N) +1.2 -2.8 N.D. +0.0 -0.2
carbon source carbon source Bisabolol 45 mM -1.2 +0.9 N.D. -1.4 +0.3
nitrogen source NAG (N); with MOPS -2.3 +1.2 N.D. -0.8 +0.6
nitrogen source D-2-Aminobutyric (N) +0.4 -2.3 N.D. +0.1 +0.7
carbon source acetate (C) +0.5 +0.7 N.D. -2.0 +0.4
micoeukaryotes Tetrahymena sp., Mixed culture-2 +1.1 +0.5 N.D. -1.9 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +1.2 +0.6 N.D. -1.3 +0.3
phage P. putida Sci Big MOI 0.1 +1.3 +0.9 N.D. -1.5 +0.6
phage P. putida Sci small MOI 0.1 +1.8 +1.0 N.D. +0.7 +0.4
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