Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5568 and PP_5569 are separated by 33 nucleotidesPP_5569 and PP_5571 are separated by 154 nucleotidesPP_5571 and PP_5572 are separated by 8 nucleotidesPP_5572 and PP_5573 are separated by 703 nucleotides PP_5568: PP_5568 - conserved membrane protein of unknown function, at 3,501,614 to 3,502,312 _5568 PP_5569: PP_5569 - protein of unknown function of phage origin, at 3,502,346 to 3,502,549 _5569 PP_5571: PP_5571 - conserved protein of unknown function, at 3,502,704 to 3,503,015 _5571 PP_5572: PP_5572 - protein of unknown function, at 3,503,024 to 3,503,305 _5572 PP_5573: PP_5573 - protein of unknown function, at 3,504,009 to 3,504,185 _5573
Group Condition PP_5568 PP_5569 PP_5571 PP_5572 PP_5573
phage P. put South MOI 10 -0.7 +0.4 -6.2 +0.8 N.D.
carbon source Myristic (C) +0.3 -0.5 -5.2 +0.6 N.D.
pH Growth at pH7 and (C) Trisodium citrate +0.7 -0.1 -5.6 +0.3 N.D.
temperature shift Temperature shift 10_to_25; with MOPS +0.2 +0.0 -2.9 -1.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -1.9 -0.7 -0.4 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.5 -3.7 -0.2 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 +0.1 -1.9 -1.5 N.D.
carbon source ferulate (C); with MOPS -0.2 +0.4 -3.2 -0.7 N.D.
phage P. putida PUT 7 MOI 1 +1.2 -0.1 -3.7 -0.6 N.D.
carbon source Octanoic (C) +0.2 +0.0 -3.7 +0.2 N.D.
temperature shift Temperature shift 10_to_30; with MOPS +0.2 -0.1 -3.2 +0.1 N.D.
nitrogen source Ammonium chloride (N) -0.1 +0.5 -2.5 -0.8 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 +0.8 -3.1 -0.5 N.D.
pH Growth at pH9 and (C) Trisodium citrate -0.8 +0.3 -2.9 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.5 -2.2 -0.7 N.D.
nitrogen source D-2-Aminobutyric (N) -0.3 -0.7 +1.2 -2.8 N.D.
temperature shift Temperature shift 10_to_30; with MOPS +0.4 -0.2 -3.3 +0.6 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.4 -2.4 -0.3 +0.8 N.D.
carbon source Tween 20 (C) -1.7 +1.2 -1.2 -0.2 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 -2.3 +1.0 +0.3 N.D.
phage PUT8 MOI 0.1 +0.5 +1.0 -2.5 -0.3 N.D.
nitrogen source 2,6-diaminopimelic (N) +0.0 +0.8 -2.5 +0.4 N.D.
nitrogen source NAG (N); with MOPS +0.5 +0.9 -2.3 +1.2 N.D.
carbon source D-Glucose (C) +0.2 -1.5 +1.2 +0.6 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.7 -0.4 +2.2 -0.5 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 +1.6 -1.2 +0.8 N.D.
phage PUT 8 MOI 1 +0.9 +1.4 -0.3 +1.1 N.D.
phage PUT8 MOI 10 +0.3 +1.7 +1.3 +0.4 N.D.
phage PUT8 MOI 10 +0.9 +1.4 +1.4 +0.1 N.D.
phage P. putida Sci small MOI 0.1 +1.1 +0.4 +1.8 +1.0 N.D.
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