Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5565 and PP_5566 are separated by 216 nucleotidesPP_5566 and PP_5567 are separated by 485 nucleotidesPP_5567 and PP_5568 are separated by 155 nucleotidesPP_5568 and PP_5569 are separated by 33 nucleotides PP_5565: PP_5565 - conserved protein of unknown function, at 3,500,053 to 3,500,325 _5565 PP_5566: PP_5566 - protein of unknown function, at 3,500,542 to 3,500,781 _5566 PP_5567: PP_5567 - protein of unknown function, at 3,501,267 to 3,501,458 _5567 PP_5568: PP_5568 - conserved membrane protein of unknown function, at 3,501,614 to 3,502,312 _5568 PP_5569: PP_5569 - protein of unknown function of phage origin, at 3,502,346 to 3,502,549 _5569
Group Condition PP_5565 PP_5566 PP_5567 PP_5568 PP_5569
micoeukaryotes Tetrahymena sp., Mixed culture-2 -2.2 -1.0 N.D. +0.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.4 +0.2 N.D. -0.4 -2.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.1 +0.7 N.D. +0.5 -3.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.0 +0.4 N.D. -0.5 -2.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.1 N.D. -0.8 -1.9
nitrogen source Urea (N); with MOPS -0.7 -0.7 N.D. +0.2 -0.7
pH Growth at pH6 and (C) Trisodium citrate -0.3 +0.5 N.D. -0.1 -1.5
carbon source D-Glucose (C) +0.2 -0.1 N.D. +0.2 -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.3 +1.0 N.D. -0.4 -1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.6 -0.6 N.D. -0.1 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.9 +0.6 N.D. -0.4 +0.1
pH pH 5 -0.2 +0.6 N.D. +0.4 -1.1
pH Growth at pH6 and (C) D-Glucose +0.3 -1.2 N.D. +0.3 +0.4
carbon source Tween 20 (C) +0.6 -0.3 N.D. -1.7 +1.2
carbon source Crotonic (C) -0.4 +0.6 N.D. -0.8 +0.6
carbon source ferulate (C); with MOPS +0.2 +0.9 N.D. -0.1 -0.9
nitrogen source NAG (N); with MOPS -1.1 -0.2 N.D. +0.5 +0.9
nitrogen source nitrate (N) -0.2 -0.5 N.D. +0.3 +1.0
phage PUT8 MOI 0.1 +0.1 -0.7 N.D. +0.3 +1.2
temperature shift Temperature shift 10_to_30; with MOPS -0.4 -0.2 N.D. +0.6 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 +1.0 N.D. +0.2 +0.3
stress Growth with Chloride 200 mM -0.5 +0.3 N.D. +0.7 +1.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 -0.3 N.D. -0.1 +1.6
temperature Growth at 25C; with MOPS -0.9 +0.6 N.D. +1.0 +1.0
temperature shift Temperature shift 30_to_25; with MOPS +0.3 -0.1 N.D. +0.6 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +0.3 N.D. +0.5 +0.7
phage P. putida Sci small MOI 0.1 -1.0 +0.6 N.D. +1.1 +1.4
phage PUT8 MOI 10 +0.5 -0.2 N.D. +0.3 +1.7
phage PUT 8 MOI 1 +0.2 +0.1 N.D. +0.9 +1.4
phage PUT8 MOI 10 +0.6 -0.2 N.D. +0.9 +1.4
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