Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3032 and PP_5552 are separated by 3 nucleotidesPP_5552 and PP_5553 overlap by 4 nucleotidesPP_5553 and PP_3033 are separated by 107 nucleotidesPP_3033 and PP_3034 are separated by 78 nucleotides PP_3032: PP_3032 - DNA-binding protein Roi-related protein, at 3,418,570 to 3,418,860 _3032 PP_5552: PP_5552 - conserved protein of unknown function, at 3,418,864 to 3,419,175 _5552 PP_5553: PP_5553 - conserved protein of unknown function, at 3,419,172 to 3,419,345 _5553 PP_3033: PP_3033 - putative transcriptional repressor, CI/C2 family, at 3,419,453 to 3,420,184 _3033 PP_3034: PP_3034 - putative Regulator protein, at 3,420,263 to 3,420,526 _3034
Group Condition PP_3032 PP_5552 PP_5553 PP_3033 PP_3034
phage P. putida PUT 7 MOI 1 N.D. -1.2 -2.3 N.D. -1.5
pH Growth at pH6 and (C) D-Glucose N.D. -3.1 +0.2 N.D. -0.7
pH Growth at pH6 and (C) D-Glucose N.D. -1.5 +0.1 N.D. -1.4
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.2 +0.1 N.D. -2.6
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. -1.1 -0.1 N.D. -1.5
nitrogen source no extra (N); with MOPS N.D. -0.1 -0.5 N.D. -2.0
temperature shift Temperature shift 30_to_34 N.D. -1.8 -0.2 N.D. -0.5
pH pH 9 N.D. -0.8 -0.1 N.D. -1.5
r2a control with 0.2% methanol R2A control with 0.2% methanol N.D. -2.3 -0.3 N.D. +0.2
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. -0.9 +0.4 N.D. -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -1.9 -0.4 N.D. +0.2
temperature shift Temperature shift 30_to_21 N.D. -1.1 +0.1 N.D. -1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.7 +0.1 N.D. -1.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.8 +0.3 N.D. -0.4
carbon source carbon source Bisabolene 48.9 mM N.D. -0.7 +0.3 N.D. -1.5
pH Growth at pH6 and (C) Trisodium citrate N.D. -1.7 -0.3 N.D. +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -2.1 +0.7 N.D. -0.2
pH Growth at pH9 and (C) Trisodium citrate N.D. -2.2 +0.3 N.D. +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.6 -0.4 N.D. -1.4
carbon source 3-methyl-1-butanol 0.25 vol% (C) N.D. -1.0 +0.6 N.D. -0.7
phage P. putida Sci small MOI 0.1 N.D. +0.7 -0.5 N.D. -1.1
temperature shift Temperature shift 10_to_34; with MOPS N.D. +0.8 -0.2 N.D. -1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.2 -0.3 N.D. +0.7
temperature Growth at 25C N.D. +0.8 -0.3 N.D. -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. +0.8 -0.5 N.D. -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.8 -0.6 N.D. +1.0
nitrogen source D-2-Aminobutyric (N) N.D. -1.3 +0.4 N.D. +0.4
phage P. putida Sci Big MOI 0.1 N.D. -0.9 -0.5 N.D. +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 +0.4 N.D. +1.6
phage PUT 8 MOI 1 N.D. +0.6 -0.2 N.D. +1.4
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