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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2820 and PP_2821 are separated by 177 nucleotides
PP_2821 and PP_5539 are separated by 11 nucleotides
PP_5539 and PP_2823 are separated by 29 nucleotides
PP_2823 and PP_2824 are separated by 559 nucleotides
PP_2820: PP_2820 - HTH-type transcriptional regulator nfxB, at 3,218,700 to 3,219,257
_2820
PP_2821: PP_2821 - conserved exported protein of unknown function, at 3,219,435 to 3,219,839
_2821
PP_5539: PP_5539 - conserved exported protein of unknown function, at 3,219,851 to 3,221,011
_5539
PP_2823: PP_2823 - Methyl-accepting chemotaxis transducer, at 3,221,041 to 3,222,693
_2823
PP_2824: PP_2824 - Transcriptional regulator, TetR family, at 3,223,253 to 3,223,933
_2824
Group
Condition
PP
_2820
PP
_2821
PP
_5539
PP
_2823
PP
_2824
carbon source
3-methyl-1-butanol 0.1 vol% (C)
-5.5
-0.0
-0.3
-0.5
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-3.7
-0.6
+0.1
-0.7
+0.2
nitrogen source
D-2-Aminobutyric (N)
-3.2
-0.5
+0.0
-0.5
-0.1
nitrogen source
D-2-Aminobutyric (N)
-3.5
+0.1
-0.0
+0.3
-0.9
phage
P. putida Sci small MOI 0.1
-0.6
-1.9
-0.5
-0.5
-0.4
phage
PUT 8 MOI 1
-1.2
-1.3
-0.4
-0.0
-0.8
carbon source
1,3-Hexanediol (C)
-2.7
-0.1
+0.1
-0.5
-0.4
pH
Growth at pH7 and (C) D-Glucose
-2.4
-0.5
-0.1
-0.1
-0.3
carbon source
Vanillin (C)
-3.0
-0.1
+0.1
-0.2
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-2.7
+0.1
+0.2
-0.2
-0.8
nitrogen source
spermidine (N)
-2.8
-0.3
-0.0
-0.1
-0.0
carbon source
3-methyl-1-butanol (C)
-2.8
-0.0
-0.0
-0.2
-0.2
carbon source
1,3-Hexanediol (C)
-2.4
-0.2
-0.0
-0.1
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-3.1
+0.0
+0.0
+0.3
-0.2
temperature shift
Temperature shift 10_to_25; with MOPS
-2.7
-0.7
+0.0
+0.2
+0.2
carbon source
Vanillin (C)
-2.7
-0.0
+0.1
-0.4
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-2.2
+0.2
+0.2
-0.3
-0.8
carbon source
ferulate (C); with MOPS
-1.7
-0.2
+0.2
+0.1
-1.3
nitrogen source
nitrite (N)
-2.3
+0.1
-0.2
-0.5
+0.3
carbon source
Tween 20 (C)
-2.1
-0.8
-0.1
+0.0
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-3.4
-0.1
+0.1
+0.0
+0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-2.2
+0.4
+0.1
-0.1
-0.5
carbon source
L-Lysine (C)
-2.2
-0.3
-0.1
-0.0
+0.5
pH
pH 6
-2.6
+0.2
+0.0
-0.2
+0.7
temperature
Growth at 25C
-1.9
-0.6
-0.1
+0.5
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-2.7
+0.3
+0.1
+0.0
+0.6
phage
P. putida Sci small MOI 0.1
+1.3
-0.8
-1.0
-0.5
+0.1
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-1.7
-0.3
-0.3
+0.9
+0.6
nitrogen source
NAG (N); with MOPS
-1.8
-0.5
+0.3
+0.2
+1.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
+1.5
+0.0
+1.6
+0.7
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