Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2511 and PP_2512 overlap by 16 nucleotidesPP_2512 and PP_5535 are separated by 35 nucleotidesPP_5535 and PP_2513 are separated by 23 nucleotidesPP_2513 and PP_2514 are separated by 2 nucleotides PP_2511: PP_2511 - conserved protein of unknown function, at 2,858,770 to 2,859,183 _2511 PP_2512: PP_2512 - GTP cyclohydrolase 1, at 2,859,168 to 2,859,728 _2512 PP_5535: PP_5535 - conserved membrane protein of unknown function, at 2,859,764 to 2,860,222 _5535 PP_2513: PP_2513 - 4-oxalomesaconate tautomerase, at 2,860,246 to 2,861,331 _2513 PP_2514: PP_2514 - 4-carboxy-4-hydroxy-2-oxoadipic acid aldolase, at 2,861,334 to 2,862,050 _2514
Group Condition PP_2511 PP_2512 PP_5535 PP_2513 PP_2514
nitrogen source NAG (N); with MOPS -1.0 -0.5 -1.1 -1.2 -0.4
phage P. putida PUT 7 MOI 1 -4.5 -0.1 +0.3 -0.2 +0.7
carbon source Valerolactone (C) -1.9 -0.2 -0.7 -0.4 -0.3
phage P. putida Sci small MOI 0.1 -5.3 +0.7 +0.6 +0.4 +0.1
carbon source Trisodium citrate (C) -1.3 -1.1 +0.2 -0.9 -0.1
pH pH 9 -2.4 +0.0 +0.2 -0.8 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 -0.4 -0.3 -0.6 -0.7
carbon source Tween 20 (C) -1.2 -0.4 -0.4 -0.6 +0.0
carbon source D-Glucose (C); with MOPS -0.8 -0.3 -0.5 -0.4 -0.5
carbon source carbon source 4-vinylphenol 0.01 mM -0.7 -0.1 +0.2 -2.2 +0.3
pH Valerolactone (C) pH 6.5 -0.3 +0.1 -0.4 -1.6 -0.1
carbon source acetate (C) -1.1 +0.2 -0.1 -1.3 +0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM -2.2 +0.0 +0.2 +0.5 -0.7
carbon source carbon source D-Glucose 55.5 mM +0.6 -0.2 -0.8 -1.2 -0.3
temperature shift Temperature shift 10_to_25; with MOPS +0.1 -0.7 +0.2 -1.4 -0.1
temperature Growth at 30C -1.4 -0.3 +0.3 -0.6 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.2 +0.4 -0.8 +0.1 -0.1
carbon source carbon source 4-vinylphenol 0.1 mM -0.4 -0.3 +0.1 -1.5 +0.5
pH Growth at pH6 and (C) D-Glucose +0.1 -0.7 +0.5 -1.0 -0.3
pH D-Glucose (C) pH 6.5 +0.0 +0.3 -0.2 -1.8 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 +0.3 -0.2 -1.1 +0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.1 +0.2 -0.0 +0.5 +0.5
nitrogen source 2,6-diaminopimelic (N) +0.6 -0.5 +0.1 +0.3 -1.4
nitrogen source D-2-Aminobutyric (N) +1.2 -0.3 -0.8 -1.4 +0.9
pH pH 7 +0.5 +0.3 -0.1 -1.4 +0.3
supernatant Supernatant; Paenibacillus sp. UNCCL52 spent media 0.5x +0.9 +0.0 -0.6 -0.4 +0.6
nitrogen source D-2-Aminobutyric (N) +1.6 -0.3 +0.5 -0.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.5 +0.5 -0.0 +1.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml +1.4 +0.6 +0.3 +0.5 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.4 -0.1 +1.5 +1.6
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