Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5490 and PP_1936 are separated by 162 nucleotidesPP_1936 and PP_5491 are separated by 2 nucleotidesPP_5491 and PP_5492 overlap by 1 nucleotidesPP_5492 and PP_1937 are separated by 57 nucleotides PP_5490: PP_5490 - site-specific DNA recombinase, at 2,183,943 to 2,184,509 _5490 PP_1936: PP_1936 - conserved membrane protein of unknown function, at 2,184,672 to 2,187,608 _1936 PP_5491: PP_5491 - conserved protein of unknown function with SEC-C motif domain, at 2,187,611 to 2,188,525 _5491 PP_5492: PP_5492 - conserved protein of unknown function, at 2,188,525 to 2,189,076 _5492 PP_1937: PP_1937 - putative helicase, at 2,189,134 to 2,192,391 _1937
Group Condition PP_5490 PP_1936 PP_5491 PP_5492 PP_1937
temperature Growth at 30C; with MOPS -0.1 -0.2 -3.5 -0.9 -0.1
nitrogen source NAG (N); with MOPS -0.8 -0.2 -2.3 +0.2 +0.2
carbon source carbon source 4-vinylphenol 0.01 mM -0.0 +0.2 -2.7 +0.0 -0.2
nitrogen source Urea (N); with MOPS -0.4 -0.4 -1.6 -0.3 +0.2
temperature shift Temperature shift 10_to_30; with MOPS -1.3 -0.3 -0.8 -0.1 +0.1
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.1 +0.1 -2.4 -0.0 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.0 +0.1 -2.2 -0.1 +0.0
pH Growth at pH7 and (C) D-Glucose -0.7 +0.2 -0.9 -0.7 +0.1
pH pH 6 +0.2 +0.2 -1.9 -0.2 -0.2
nitrogen source D-2-Aminobutyric (N) +0.1 -0.5 -1.6 +0.1 +0.1
nitrogen source Urea (N) +0.4 -0.3 -1.1 -0.6 -0.0
pH pH 4 -0.3 +0.4 -1.2 -0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 -0.0 -1.2 +0.7 -0.0
nitrogen source no extra (N); with MOPS +0.2 +0.2 -1.7 +0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +0.1 -1.4 +0.5 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.2 -0.6 +0.8 -0.9 -0.1
pH pH 7 +0.5 +0.5 -1.1 -0.1 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.3 +0.4 +0.5 -0.8 +0.1
pH Growth at pH7 and (C) D-Glucose -0.5 -0.0 +1.4 +0.2 +0.0
phage PUT8 MOI 10 +0.7 +0.4 +0.7 -0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 -0.1 +0.8 +0.2 -0.0
phage PUT8 MOI 10 +0.2 +0.2 +1.3 -0.1 +0.3
nitrogen source 2,6-diaminopimelic (N) +0.2 +0.3 +0.8 +0.5 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.6 +0.0 +1.8 -0.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 +0.8 +0.8 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.5 +0.2 +1.0 +0.6 +0.0
phage P. putida Sci small MOI 0.1 +1.0 +0.3 +0.2 +0.1 +0.6
phage P. putida Missley MOI 0.1 +0.7 +0.6 +0.5 +0.5 +0.9
phage P. putida PUT 7 MOI 1 +0.5 +0.6 +0.5 +0.6 +1.0
phage P. putida Sci small MOI 0.1 +0.6 +0.5 +1.6 +0.6 +0.5
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