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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PP_5490 and PP_1936 are separated by 162 nucleotides
PP_1936 and PP_5491 are separated by 2 nucleotides
PP_5491 and PP_5492 overlap by 1 nucleotides
PP_5492 and PP_1937 are separated by 57 nucleotides
PP_5490: PP_5490 - site-specific DNA recombinase, at 2,183,943 to 2,184,509
_5490
PP_1936: PP_1936 - conserved membrane protein of unknown function, at 2,184,672 to 2,187,608
_1936
PP_5491: PP_5491 - conserved protein of unknown function with SEC-C motif domain, at 2,187,611 to 2,188,525
_5491
PP_5492: PP_5492 - conserved protein of unknown function, at 2,188,525 to 2,189,076
_5492
PP_1937: PP_1937 - putative helicase, at 2,189,134 to 2,192,391
_1937
Group
Condition
PP
_5490
PP
_1936
PP
_5491
PP
_5492
PP
_1937
temperature
Growth at 30C; with MOPS
-0.1
-0.2
-3.5
-0.9
-0.1
nitrogen source
NAG (N); with MOPS
-0.8
-0.2
-2.3
+0.2
+0.2
carbon source
carbon source 4-vinylphenol 0.01 mM
-0.0
+0.2
-2.7
+0.0
-0.2
nitrogen source
Urea (N); with MOPS
-0.4
-0.4
-1.6
-0.3
+0.2
temperature shift
Temperature shift 10_to_30; with MOPS
-1.3
-0.3
-0.8
-0.1
+0.1
r2a control with 0.2% methanol
R2A control with 0.2% methanol
+0.1
+0.1
-2.4
-0.0
-0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.0
+0.1
-2.2
-0.1
+0.0
pH
Growth at pH7 and (C) D-Glucose
-0.7
+0.2
-0.9
-0.7
+0.1
pH
pH 6
+0.2
+0.2
-1.9
-0.2
-0.2
nitrogen source
D-2-Aminobutyric (N)
+0.1
-0.5
-1.6
+0.1
+0.1
nitrogen source
Urea (N)
+0.4
-0.3
-1.1
-0.6
-0.0
pH
pH 4
-0.3
+0.4
-1.2
-0.1
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.6
-0.0
-1.2
+0.7
-0.0
nitrogen source
no extra (N); with MOPS
+0.2
+0.2
-1.7
+0.2
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.4
+0.1
-1.4
+0.5
+0.1
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.2
-0.6
+0.8
-0.9
-0.1
pH
pH 7
+0.5
+0.5
-1.1
-0.1
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.3
+0.4
+0.5
-0.8
+0.1
pH
Growth at pH7 and (C) D-Glucose
-0.5
-0.0
+1.4
+0.2
+0.0
phage
PUT8 MOI 10
+0.7
+0.4
+0.7
-0.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+1.0
-0.1
+0.8
+0.2
-0.0
phage
PUT8 MOI 10
+0.2
+0.2
+1.3
-0.1
+0.3
nitrogen source
2,6-diaminopimelic (N)
+0.2
+0.3
+0.8
+0.5
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.6
+0.0
+1.8
-0.4
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.4
+0.8
+0.8
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.5
+0.2
+1.0
+0.6
+0.0
phage
P. putida Sci small MOI 0.1
+1.0
+0.3
+0.2
+0.1
+0.6
phage
P. putida Missley MOI 0.1
+0.7
+0.6
+0.5
+0.5
+0.9
phage
P. putida PUT 7 MOI 1
+0.5
+0.6
+0.5
+0.6
+1.0
phage
P. putida Sci small MOI 0.1
+0.6
+0.5
+1.6
+0.6
+0.5
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