Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1807 and PP_1808 are separated by 275 nucleotidesPP_1808 and PP_5477 are separated by 1440 nucleotidesPP_5477 and PP_1810 are separated by 651 nucleotidesPP_1810 and PP_5478 are separated by 198 nucleotides PP_1807: PP_1807 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,031,402 to 2,032,244 _1807 PP_1808: PP_1808 - glucose-6-phosphate isomerase, at 2,032,520 to 2,034,184 _1808 PP_5477: PP_5477 - conserved protein of unknown function, at 2,035,625 to 2,036,014 _5477 PP_1810: PP_1810 - conserved protein of unknown function, at 2,036,666 to 2,037,406 _1810 PP_5478: PP_5478 - conserved protein of unknown function, at 2,037,605 to 2,037,880 _5478
Group Condition PP_1807 PP_1808 PP_5477 PP_1810 PP_5478
carbon source Tween 20 (C) +0.1 -1.2 -0.6 +0.1 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 -2.1 -0.3 +0.5 +0.1
carbon source D-Glucose (C); with MOPS +0.4 -1.8 -0.4 -0.1 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 -1.7 +0.3 +0.2 -0.2
phage PUT8 MOI 0.1 -2.4 +0.7 +0.7 +0.2 -0.1
phage P. put PUT 10 MOI 1 -1.5 +0.9 -0.4 -0.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.0 -1.7 -0.1 +0.7 +0.2
phage PUT8 MOI 0.1 -1.7 +1.1 -0.4 +0.1 +0.3
pH Growth at pH6 and (C) D-Glucose -0.2 +0.4 +0.6 +0.3 -1.6
phage P. putida South MOI 1 -1.3 +0.5 -0.3 +0.2 +0.4
phage P. putida Sci small MOI 0.1 +0.2 -1.4 +0.4 +0.2 +0.2
phage PUT8 MOI 10 -0.9 +0.0 +0.7 +0.3 -0.5
phage P. putida PUT10 MOI 0.1 -1.4 +0.8 +0.1 -0.1 +0.4
phage P. put South MOI 0.1 -1.4 +0.8 +0.3 +0.1 +0.1
phage P. put PUT 10 MOI 1 -1.3 +0.9 +0.0 +0.1 +0.1
phage P. putida South MOI 0.1 -1.3 +0.5 +0.4 -0.0 +0.3
phage P. putida PUT10 MOI 0.1 -1.2 +0.9 -0.3 +0.2 +0.3
r2a control R2A control +0.1 -1.3 +0.5 +0.2 +0.4
phage P. put South MOI 0.1 -1.2 +0.9 +0.1 +0.2 +0.2
phage P. put South MOI 10 -0.7 +1.0 -0.0 -0.7 +0.5
phage PUT 8 MOI 1 -1.9 +1.0 +1.2 +0.0 -0.0
phage P. put South MOI 1 -1.2 +1.2 +0.6 -0.2 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 -1.2 +0.5 +0.2 +0.7
phage P. putida South MOI 0.1 -1.2 +1.0 -0.2 +0.1 +0.6
nitrogen source NAG (N); with MOPS +0.1 +0.5 +0.8 +0.5 -1.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +1.1 -0.6 +0.1 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 +0.9 +1.1 -0.6 -0.5
phage P. putida South MOI 10 -0.7 +0.9 -0.1 +0.5 +0.3
phage PUT8 MOI 10 -0.9 +0.8 +0.6 -0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.6 +0.3 -0.4 +1.1
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