Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1410 and PP_1411 overlap by 29 nucleotidesPP_1411 and PP_5470 are separated by 212 nucleotidesPP_5470 and PP_1412 are separated by 44 nucleotidesPP_1412 and PP_1413 are separated by 126 nucleotides PP_1410: PP_1410 - conserved protein of unknown function, at 1,608,578 to 1,608,811 _1410 PP_1411: PP_1411 - GGDEF domain protein, at 1,608,783 to 1,610,264 _1411 PP_5470: PP_5470 - conserved protein of unknown function, at 1,610,477 to 1,610,926 _5470 PP_1412: PP_1412 - 3-hydroxyisobutyryl-CoA hydrolase, at 1,610,971 to 1,612,077 _1412 PP_1413: PP_1413 - Uracil-DNA glycosylase, at 1,612,204 to 1,612,896 _1413
Group Condition PP_1410 PP_1411 PP_5470 PP_1412 PP_1413
micoeukaryotes Tetrahymena sp., Mixed culture-2 -5.6 -0.0 +0.2 -0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -5.2 +0.3 -0.0 -0.2 -0.3
nitrogen source NAG (N); with MOPS -4.3 -0.1 +0.3 -0.9 +0.1
temperature shift Temperature shift 30_to_34; with MOPS -4.3 -0.0 +0.0 -0.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -4.3 +0.1 +0.0 -0.4 +0.2
nitrogen source Propandiamine (N) -4.3 +0.1 +0.0 -0.1 +0.1
pH Growth at pH6 and (C) D-Glucose -4.4 -0.1 +0.1 +0.1 +0.2
phage P. putida Sci small MOI 0.1 -3.7 +0.2 +0.1 +0.2 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -3.4 +0.0 -0.1 -0.3 -0.0
phage P. putida Missley MOI 0.1 -3.1 -0.0 -0.1 +0.1 -0.6
carbon source ferulate (C); with MOPS -4.0 +0.2 +0.0 -0.2 +0.4
nitrogen source 2,6-diaminopimelic (N) -3.3 -0.1 +0.4 -0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.8 -0.2 -0.0 -0.1 -0.2
pH Growth at pH7 and (C) Trisodium citrate -2.7 -0.3 +0.1 -0.4 +0.2
pH Growth at pH8 and (C) Trisodium citrate -2.8 +0.0 +0.0 -0.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.6 +0.1 -0.2 +0.1 -0.1
phage P. putida Sci Big MOI 0.1 -2.8 +0.0 +0.4 -0.2 +0.1
micoeukaryotes C.elegans, mixed culture-2 -2.4 -0.1 +0.3 +0.1 -0.0
micoeukaryotes Acanthamoeba sp., muxed culture-1 -1.8 -0.4 +0.3 +0.3 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.5 -0.1 +0.1 -0.6 +0.9
pH pH 6 -1.5 +0.1 +0.4 +0.4 +0.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.1 +0.1 +1.2 +0.6 +0.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 +0.1 +1.3 +0.4 -0.3
stress R2A with Fusaric 240 ug/mL +2.0 -0.2 -0.1 -0.5 +0.3
no stress control Growth with no stress +2.2 -0.3 +0.1 -0.2 -0.1
carbon source D-Glucose (C) +1.7 +0.2 +0.1 -0.5 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 -0.0 +0.2 -0.4 +0.8
temperature shift Temperature shift 30_to_25 +2.2 +0.0 -0.0 +0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.9 +0.2 +0.1 +0.1 -0.2
stress R2A with Polymyxin B sulfate 0.001 mg/ml +3.4 -0.1 +0.0 -0.0 -0.1
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