Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5464 and PP_1116 are separated by 204 nucleotidesPP_1116 and PP_5465 are separated by 594 nucleotidesPP_5465 and PP_1117 are separated by 562 nucleotidesPP_1117 and PP_5742 are separated by 606 nucleotides PP_5464: PP_5464 - protein of unknown function, at 1,276,017 to 1,276,454 _5464 PP_1116: PP_1116 - Site-specific recombinase, resolvase family, at 1,276,659 to 1,277,369 _1116 PP_5465: PP_5465 - conserved protein of unknown function, at 1,277,964 to 1,278,173 _5465 PP_1117: PP_1117 - conserved protein of unknown function, at 1,278,736 to 1,279,995 _1117 PP_5742: PP_5742 - putative membrane protein of unknown function, at 1,280,602 to 1,281,174 _5742
Group Condition PP_5464 PP_1116 PP_5465 PP_1117 PP_5742
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.0 -1.7 -0.2 +0.2 +0.1
nitrogen source 2,6-diaminopimelic (N) -0.0 -0.9 -1.1 +0.8 +0.0
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay +0.1 -0.2 -1.1 +0.4 -0.2
pH Valerolactone (C) pH 6.5 +0.3 -0.1 -0.7 +0.3 -0.6
nitrogen source L-Phenylalanine (N) +0.0 +0.2 -1.3 +0.2 +0.3
nitrogen source nitrate (N) +0.2 -0.7 -0.4 +0.3 +0.3
nitrogen source 2,6-diaminopimelic (N) +0.3 -1.0 -0.3 +0.6 +0.1
nitrogen source Propiolactam (N) +0.4 +0.0 -1.0 +0.5 -0.1
pH Valerolactone (C) pH 6.5 -0.0 +0.6 -0.7 +0.4 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.2 +0.5 +0.2 +0.2 -0.6
carbon source L-Lysine (C) +0.3 +0.7 -0.3 -0.2 -0.4
nitrogen source Propiolactam (N) +0.2 -0.0 -0.9 +0.7 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.1 -0.4 -0.5 +0.6 +0.4
nitrogen source nitrate (N) +0.1 +0.1 -0.7 +0.2 +0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 -0.1 -0.5 -0.1 +0.7
carbon source 3-methyl-1-butanol 0.25 vol% (C) +0.6 -0.2 +0.5 +0.1 -0.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +0.3 +0.1 -0.7 +0.9 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.6 +0.9 +0.2 +0.0
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +0.3 +0.3 -0.4 +0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.8 +0.4 -0.1 +0.3
nitrogen source D-2-Aminobutyric (N) +0.4 +0.8 +0.5 -0.1 +0.2
phage PUT8 MOI 10 +0.2 +0.5 +0.5 +0.4 +0.3
phage P. putida compost MOI 0.1 +0.4 +0.2 +0.5 +0.3 +0.5
phage PUT8 MOI 0.1 +0.3 +0.5 +0.4 +0.2 +0.6
phage PUT8 MOI 10 +0.3 +0.6 +0.8 +0.1 +0.7
phage P. putida Sci Big MOI 0.1 +0.4 +0.3 +1.0 +0.9 +0.1
phage P. putida Missley MOI 0.1 +0.6 +0.7 +1.2 +0.9 +0.7
phage P. putida PUT 7 MOI 1 +0.8 +0.7 +1.1 +0.6 +0.9
phage P. putida Sci small MOI 0.1 +0.9 +0.2 +0.9 +0.9 +1.3
phage P. putida Sci small MOI 0.1 +0.9 +0.7 +1.1 +0.8 +1.3
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