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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0384 and PP_0385 are separated by 187 nucleotides
PP_0385 and PP_5445 are separated by 343 nucleotides
PP_5445 and PP_t71 are separated by 52 nucleotides
PP_t71 and PP_0386 are separated by 16 nucleotides
PP_0384: PP_0384 - Transcriptional regulator, AsnC family, at 465,350 to 465,811
_0384
PP_0385: PP_0385 - putative serine acetyltransferase, at 465,999 to 466,553
_0385
PP_5445: PP_5445 - conserved protein of unknown function, at 466,897 to 467,067
_5445
PP_t71: PP_t71 - tRNA-Met, at 467,120 to 467,196
_t71
PP_0386: PP_0386 - Sensory box protein, at 467,213 to 470,956
_0386
Group
Condition
PP
_0384
PP
_0385
PP
_5445
PP
_t71
PP
_0386
carbon source
L-Lysine (C)
-5.1
+0.0
-0.5
N.D.
+0.2
carbon source
L-Lysine (C)
-5.7
+0.1
+0.3
N.D.
+0.3
nitrogen source
L-Lysine (N)
-3.3
+0.1
-0.4
N.D.
+0.0
carbon source
L-Lysine (C)
-3.0
-0.2
-0.5
N.D.
+0.2
carbon source
L-Lysine 10 mM (C)
-2.5
-0.2
-0.6
N.D.
+0.1
nitrogen source
L-Lysine (N)
-3.0
+0.1
-0.3
N.D.
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
-0.2
-0.3
-2.3
N.D.
+0.0
carbon source
ferulate (C); with MOPS
+0.2
+0.3
-3.2
N.D.
+0.0
carbon source
3-Hydroxydecanoic (C)
-0.0
-0.2
-2.3
N.D.
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.2
-0.5
-1.2
N.D.
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.2
-0.1
-1.7
N.D.
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.0
+0.3
-2.1
N.D.
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.5
-0.2
-1.4
N.D.
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.0
+0.4
-0.7
N.D.
-1.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.0
+0.4
-1.4
N.D.
-0.3
carbon source
L-Leucine (C)
+0.3
-0.1
-1.8
N.D.
+0.4
carbon source
L-Leucine (C)
+0.2
+0.2
-1.6
N.D.
+0.2
phage
P. put PUT 10 MOI 1
+0.2
+0.3
-1.7
N.D.
+0.2
carbon source
ferulate (C); with MOPS
+0.3
-1.3
+0.4
N.D.
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
-0.7
+1.3
N.D.
-0.1
pH
pH 9
+0.2
+1.0
-0.9
N.D.
+0.1
pH
Valerolactone (C) pH 6.5
+0.5
-0.6
+0.9
N.D.
-0.3
pH
Growth at pH7 and (C) Trisodium citrate
+0.2
-0.3
+1.2
N.D.
-0.3
micoeukaryotes
Acanthamoeba sp., muxed culture-1
+1.2
-0.1
-0.3
N.D.
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.3
+0.3
+1.5
N.D.
+0.1
nitrogen source
NAG (N); with MOPS
+0.8
+0.3
+1.0
N.D.
-0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.4
-0.1
+1.6
N.D.
+0.1
nitrogen source
NAG (N); with MOPS
+0.1
+0.7
+1.1
N.D.
+0.3
carbon source
carbon source 4-vinylphenol 0.01 mM
+0.5
+1.1
+0.8
N.D.
+0.0
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+1.1
+2.5
N.D.
+0.3
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