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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5410 and PP_5411 are separated by 189 nucleotides
PP_5411 and PP_5412 are separated by 118 nucleotides
PP_5412 and PP_5413 are separated by 43 nucleotides
PP_5413 and PP_5414 are separated by 27 nucleotides
PP_5410: PP_5410 - Transcriptional regulator, DeoR family, at 6,172,305 to 6,173,081
_5410
PP_5411: PP_5411 - N-acetyl glucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyl transferase, at 6,173,271 to 6,174,638
_5411
PP_5412: PP_5412 - ATP synthase epsilon chain, at 6,174,757 to 6,175,176
_5412
PP_5413: PP_5413 - ATP synthase subunit beta, at 6,175,220 to 6,176,596
_5413
PP_5414: PP_5414 - ATP synthase gamma chain, at 6,176,624 to 6,177,484
_5414
Group
Condition
PP
_5410
PP
_5411
PP
_5412
PP
_5413
PP
_5414
temperature shift
Temperature shift 10_to_30
-0.7
N.D.
N.D.
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.6
N.D.
N.D.
N.D.
N.D.
phage
PUT 8 MOI 1
-0.6
N.D.
N.D.
N.D.
N.D.
nitrogen source
Urea (N)
-0.6
N.D.
N.D.
N.D.
N.D.
pH
Growth at pH7 and (C) Trisodium citrate
-0.6
N.D.
N.D.
N.D.
N.D.
carbon source
Valeric (C)
-0.6
N.D.
N.D.
N.D.
N.D.
nitrogen source
D-2-Aminobutyric (N)
-0.6
N.D.
N.D.
N.D.
N.D.
carbon source
D-Mannose (C)
-0.5
N.D.
N.D.
N.D.
N.D.
pH
Growth at pH6 and (C) D-Glucose
-0.5
N.D.
N.D.
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.5
N.D.
N.D.
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.5
N.D.
N.D.
N.D.
N.D.
carbon source
Nonanoic (C)
-0.5
N.D.
N.D.
N.D.
N.D.
nitrogen source
Ammonium chloride and TNT (N)
-0.5
N.D.
N.D.
N.D.
N.D.
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.5
N.D.
N.D.
N.D.
N.D.
nitrogen source
TNT (N)
-0.5
N.D.
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
N.D.
N.D.
N.D.
N.D.
carbon source
ferulate (C); with MOPS
-0.4
N.D.
N.D.
N.D.
N.D.
pH
D-Glucose (C) pH 7.5
-0.4
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.4
N.D.
N.D.
N.D.
N.D.
phage
P. putida Sci Big MOI 0.1
-0.4
N.D.
N.D.
N.D.
N.D.
pH
Growth at pH7 and (C) D-Glucose
-0.4
N.D.
N.D.
N.D.
N.D.
carbon source
ferulate (C); with MOPS
+0.4
N.D.
N.D.
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.4
N.D.
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
N.D.
N.D.
N.D.
N.D.
carbon source
L-Lysine (C)
+0.5
N.D.
N.D.
N.D.
N.D.
phage
P. putida PUT 7 MOI 1
+0.5
N.D.
N.D.
N.D.
N.D.
pH
Growth at pH7 and (C) Trisodium citrate
+0.5
N.D.
N.D.
N.D.
N.D.
stress
Growth with Chloride 500 mM
+0.6
N.D.
N.D.
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.6
N.D.
N.D.
N.D.
N.D.
phage
P. putida Missley MOI 0.1
+0.6
N.D.
N.D.
N.D.
N.D.
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