Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5379 and PP_5732 overlap by 11 nucleotidesPP_5732 and PP_5380 are separated by 14 nucleotidesPP_5380 and PP_5381 are separated by 195 nucleotidesPP_5381 and PP_5382 are separated by 131 nucleotides PP_5379: PP_5379 - copper resistance protein B, at 6,130,442 to 6,131,521 _5379 PP_5732: PP_5732 - putative metal-binding protein, at 6,131,511 to 6,131,828 _5732 PP_5380: PP_5380 - copper resistance protein A, at 6,131,843 to 6,133,852 _5380 PP_5381: PP_5381 - hypothetical protein, at 6,134,048 to 6,134,302 _5381 PP_5382: PP_5382 - putative exported protein, at 6,134,434 to 6,134,793 _5382
Group Condition PP_5379 PP_5732 PP_5380 PP_5381 PP_5382
nitrogen source Ammonium chloride and TNT (N) -0.8 -0.5 -1.1 -0.5 +0.1
nitrogen source Ammonium chloride and TNT (N) -0.6 -0.7 -1.2 -0.1 -0.0
pH Growth at pH6 and (C) D-Glucose -0.3 -1.1 -0.1 +0.1 -1.1
nitrogen source Ammonium chloride (N) -0.3 -0.6 -0.6 -0.4 -0.1
nitrogen source TNT (N) -0.4 -0.5 -0.7 -0.6 +0.2
carbon source carbon source D-Glucose 55.5 mM +0.1 -0.6 -0.0 -0.7 -0.7
nitrogen source Ammonium chloride and TNT (N) -0.8 -0.3 -1.0 -0.0 +0.1
nitrogen source Ammonium chloride and TNT (N) -0.6 -0.4 -1.1 -0.1 +0.2
pH pH 7 -0.2 -0.6 -0.1 -0.5 -0.6
nitrogen source Ammonium chloride (N) -0.4 -0.4 -0.6 -0.5 +0.0
nitrogen source TNT (N) -0.4 -0.3 -0.8 -0.3 +0.2
nitrogen source TNT (N) -0.4 -0.1 -0.8 -0.4 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.8 +0.0 -0.4 -0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.1 +0.2 -0.8 -0.8
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.3 +0.2 +0.1 -0.7 -0.7
pH Growth at pH6 and (C) Trisodium citrate +0.1 +0.1 -0.0 -0.6 -1.0
carbon source 4-Methyl-2-oxopentanoic (C) -0.1 +0.2 -0.1 -1.2 -0.3
pH Growth at pH6 and (C) Trisodium citrate -0.2 +0.4 -0.2 -0.7 -0.4
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -0.2 +0.0 +0.1 -1.2 +0.4
pH Growth at pH7 and (C) D-Glucose +0.0 +0.4 +0.0 -0.9 -0.5
pH pH 6 -0.1 -0.1 -0.1 +0.7 -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.3 +0.2 -0.1 +0.6 -0.7
no stress control Growth with no stress -0.1 +0.2 +0.1 -0.7 +0.7
temperature Growth at 25C -0.1 +0.3 -0.0 -0.6 +0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +1.1 -0.2 -0.7 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -0.6 -0.2 +1.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 -0.4 +0.1 +1.0 +0.3
phage P. putida PUT 7 MOI 1 +0.4 +0.3 +0.3 -0.2 +0.7
phage P. putida Missley MOI 0.1 +0.4 +0.5 +0.5 +0.8 +0.7
carbon source Delta-Nonalactone (C) +0.7 +0.4 +1.8 -0.1 +0.5
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