Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5730 and PP_5350 are separated by 212 nucleotidesPP_5350 and PP_5351 are separated by 88 nucleotidesPP_5351 and PP_5352 are separated by 214 nucleotidesPP_5352 and PP_5353 are separated by 132 nucleotides PP_5730: PP_5730 - conserved protein of unknown function, at 6,097,791 to 6,098,009 _5730 PP_5350: PP_5350 - Transcriptional regulator, RpiR family, at 6,098,222 to 6,099,088 _5350 PP_5351: PP_5351 - glucose-6-phosphate 1-dehydrogenase, at 6,099,177 to 6,100,634 _5351 PP_5352: PP_5352 - DNA-dependent ATPase I and helicase II, nucleotide Excision Repair Complex, at 6,100,849 to 6,103,035 _5352 PP_5353: PP_5353 - conserved exported protein of unknown function, at 6,103,168 to 6,104,022 _5353
Group Condition PP_5730 PP_5350 PP_5351 PP_5352 PP_5353
stress Formaldehyde 0.03125 vol% -0.3 -1.2 +0.1 -3.4 -0.3
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM -0.3 -4.0 -0.1 -0.5 +0.2
temperature shift Temperature shift 10_to_25; with MOPS -0.3 -4.2 +0.0 +0.3 -0.6
nitrogen source DL-2-Aminobutyric (N) -0.1 -4.3 +0.5 -0.5 -0.2
stress MOPS minimal media_Glucose20mM with Chloride 200 mM -0.1 -3.5 -0.1 -0.6 -0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 -2.2 -0.3 -0.9 -1.0
stress MOPS minimal media_Glucose20mM with Lithium chloride 200 mM -0.2 -3.9 +0.1 -0.5 -0.1
stress Growth with Chloride 500 mM; with MOPS -0.4 -3.2 -0.2 -0.4 -0.3
pH Growth at pH8 and (C) D-Glucose -0.5 -4.2 -0.2 -0.1 +0.5
carbon source Delta-Undecalactone (C) +0.2 -2.7 -0.1 -0.7 -0.8
stress Growth with Chloride 500 mM +0.2 -3.6 -0.1 -0.2 -0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.0 -1.8 -0.1 -1.4 -1.6
pH D-Glucose (C) pH 6 +0.1 -2.6 -0.1 -0.6 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.4 -2.0 +0.1 -0.2 -0.4
nitrogen source DL-2-Aminobutyric (N) -0.0 -3.8 +0.6 -0.4 -0.2
temperature shift Temperature shift 30_to_25; with MOPS -0.3 -3.9 +0.1 +0.2 +0.2
nitrogen source L-Leucine (N) +0.1 -3.5 +0.1 -0.5 +0.0
nitrogen source NAG (N); with MOPS +0.4 -4.0 -0.1 -0.2 +0.3
temperature shift Temperature shift 30_to_25; with MOPS -0.0 -3.9 -0.1 +0.0 +0.4
pH D-Glucose (C) pH 6.5 +0.2 -2.9 -0.1 -0.9 +0.2
temperature Growth at 25C -1.2 -2.6 +0.0 +0.4 -0.1
nitrogen source Urea (N) -0.2 -3.2 -0.5 -0.3 +0.8
pH Growth at pH7 and (C) D-Glucose +0.2 -3.4 -0.1 -0.6 +0.5
carbon source 5-Aminovaleric 10 mM (C) +0.0 -2.7 +0.3 -0.9 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.4 -3.1 +0.4 -0.5 +0.4
carbon source D-Fructose (C) +0.5 -3.0 -0.1 -0.5 -0.1
nitrogen source D-2-Aminobutyric (N) +0.2 -3.2 +0.8 -0.1 -0.3
nitrogen source D-2-Aminobutyric (N) +0.3 -3.8 +1.0 +0.3 +0.4
phage PUT 8 MOI 1 -0.8 +2.0 -0.0 -1.0 -0.3
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +0.3 +2.0 +0.3 +1.5 +0.8
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