Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5299 and PP_5300 are separated by 28 nucleotidesPP_5300 and PP_5301 are separated by 240 nucleotidesPP_5301 and PP_5302 are separated by 59 nucleotidesPP_5302 and PP_5303 are separated by 23 nucleotides PP_5299: PP_5299 - glutamate-putrescine ligase, at 6,046,115 to 6,047,497 _5299 PP_5300: PP_5300 - Transcriptional regulator, LysR family, at 6,047,526 to 6,048,575 _5300 PP_5301: PP_5301 - DNA-directed RNA polymerase subunit omega, at 6,048,816 to 6,049,079 _5301 PP_5302: PP_5302 - bifunctional (p)ppGpp synthase/hydrolase, at 6,049,139 to 6,051,247 _5302 PP_5303: PP_5303 - aminoacrylate/iminopropionate hydrolase/deaminase, at 6,051,271 to 6,051,651 _5303
Group Condition PP_5299 PP_5300 PP_5301 PP_5302 PP_5303
pH Growth at pH6 and (C) Trisodium citrate +0.0 -0.0 N.D. N.D. -6.4
temperature shift Temperature shift 30_to_25 +0.2 +0.1 N.D. N.D. -6.6
temperature shift Temperature shift 30_to_25; with MOPS +0.1 +0.1 N.D. N.D. -6.3
pH Growth at pH7 and (C) D-Glucose +0.5 +0.2 N.D. N.D. -6.9
pH Growth at pH6 and (C) Trisodium citrate -0.3 +0.3 N.D. N.D. -6.1
nitrogen source Propandiamine (N) -0.6 -0.7 N.D. N.D. -4.6
nitrogen source Propandiamine (N) -1.0 -0.8 N.D. N.D. -4.1
carbon source Vanillin (C) -0.1 +0.0 N.D. N.D. -5.8
temperature shift Temperature shift 30_to_25 +0.3 +0.3 N.D. N.D. -6.4
carbon source Myristic (C) -0.3 +0.0 N.D. N.D. -5.5
nitrogen source Putrescine (N) -1.1 -0.4 N.D. N.D. -4.2
carbon source Vanillin (C) +0.0 -0.2 N.D. N.D. -5.5
nitrogen source D-Lysine (N) +0.0 +0.1 N.D. N.D. -5.7
carbon source Protocatechuic Acid (C) -0.0 +0.0 N.D. N.D. -5.4
nitrogen source L-Serine (N) -0.2 +0.1 N.D. N.D. -5.3
temperature shift Temperature shift 10_to_30; with MOPS +0.1 -0.2 N.D. N.D. -5.2
pH Growth at pH7 and (C) D-Glucose +0.1 +0.1 N.D. N.D. -5.6
nitrogen source Urea (N) -0.1 -0.0 N.D. N.D. -5.1
pH Growth at pH8 and (C) D-Glucose +0.3 +0.0 N.D. N.D. -5.5
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.3 -0.3 N.D. N.D. -4.7
nitrogen source Ammonium chloride (N); with MOPS -0.1 +0.0 N.D. N.D. -5.1
stress MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL +0.2 +0.0 N.D. N.D. -5.1
stress MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL +0.3 -0.1 N.D. N.D. -5.1
carbon source acetate (C) +0.1 +0.3 N.D. N.D. -5.4
stress MOPS minimal media_Glucose20mM with Chloride 200 mM +0.2 +0.0 N.D. N.D. -5.0
nitrogen source nitrite (N) +0.0 +0.1 N.D. N.D. -5.0
carbon source carbon source Bisabolene 48.9 mM -0.0 +0.2 N.D. N.D. -4.9
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM +0.2 -0.1 N.D. N.D. -4.8
temperature shift Temperature shift 10_to_25; with MOPS +0.7 -0.4 N.D. N.D. -4.7
carbon source carbon source D-Glucose 55.5 mM +0.3 +0.1 N.D. N.D. -4.6
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