Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5296 and PP_5297 are separated by 33 nucleotidesPP_5297 and PP_5298 are separated by 53 nucleotidesPP_5298 and PP_5299 are separated by 4 nucleotidesPP_5299 and PP_5300 are separated by 28 nucleotides PP_5296: PP_5296 - deoxyguanylate kinase / guanylate kinase, at 6,043,028 to 6,043,648 _5296 PP_5297: PP_5297 - putative Amino acid transporter, at 6,043,682 to 6,045,289 _5297 PP_5298: PP_5298 - putative glutamine amidotransferase, at 6,045,343 to 6,046,110 _5298 PP_5299: PP_5299 - glutamate-putrescine ligase, at 6,046,115 to 6,047,497 _5299 PP_5300: PP_5300 - Transcriptional regulator, LysR family, at 6,047,526 to 6,048,575 _5300
Group Condition PP_5296 PP_5297 PP_5298 PP_5299 PP_5300
nitrogen source Propandiamine (N) N.D. +0.3 -1.1 -1.0 -0.8
nitrogen source Putrescine (N) N.D. -0.5 -0.5 -1.1 -0.4
nitrogen source Propandiamine (N) N.D. +0.7 -1.6 -0.6 -0.7
nitrogen source spermidine (N) N.D. -0.2 -0.6 -0.8 -0.4
carbon source ferulate (C); with MOPS N.D. -0.3 -1.4 +0.1 -0.2
nitrogen source Putrescine (N) N.D. -0.4 -0.2 -0.7 -0.4
nitrogen source spermidine (N) N.D. -0.3 -0.3 -0.6 -0.2
nitrogen source D-2-Aminobutyric (N) N.D. -0.5 +0.3 -0.7 -0.4
phage P. putida Sci Big MOI 0.1 N.D. -0.2 -0.6 -0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.0 -1.1 +0.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.5 -1.2 +0.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.4 -0.9 +0.0 -0.1
carbon source ferulate (C); with MOPS N.D. -0.2 -0.8 +0.5 +0.1
temperature shift Temperature shift 10_to_25; with MOPS N.D. -0.2 -0.6 +0.4 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. +0.3 -1.2 +0.4 +0.5
carbon source Valeric (C) N.D. +0.1 +0.7 -0.3 +0.1
pH Growth at pH9 and (C) Trisodium citrate N.D. -0.2 +0.6 +0.4 -0.2
pH pH 5 N.D. -0.2 +0.8 +0.2 -0.2
nitrogen source nitrogen source nitrate 5mM N.D. +0.1 +0.3 +0.7 -0.4
temperature shift Temperature shift 10_to_25; with MOPS N.D. -0.0 +0.6 +0.7 -0.4
r2a control R2A control N.D. -0.2 +0.6 +0.3 +0.3
carbon source Hexanoic (C) N.D. -0.1 +0.7 +0.3 +0.2
pH Growth at pH7 and (C) Trisodium citrate N.D. -0.1 +0.7 +0.5 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 +1.2 +0.1 +0.0
nitrogen source NAG (N); with MOPS N.D. -0.2 +0.9 +0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +1.0 +0.2 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. +0.1 +0.8 +0.1 +0.4
carbon source L-tyrosine 5 mM (C) N.D. +0.1 +0.7 +0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.2 +0.7 +0.3 +0.5
pH Growth at pH7 and (C) D-Glucose N.D. -0.3 +1.4 +0.5 +0.2
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