Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5251 and PP_5252 are separated by 176 nucleotidesPP_5252 and PP_5253 are separated by 199 nucleotidesPP_5253 and PP_5254 are separated by 40 nucleotidesPP_5254 and PP_5255 are separated by 87 nucleotides PP_5251: PP_5251 - putative Transporter, at 5,993,274 to 5,994,872 _5251 PP_5252: PP_5252 - conserved exported protein of unknown function, at 5,995,049 to 5,997,031 _5252 PP_5253: PP_5253 - Arylesterase, at 5,997,231 to 5,998,049 _5253 PP_5254: PP_5254 - conserved protein of unknown function, at 5,998,090 to 5,998,428 _5254 PP_5255: PP_5255 - putative hydrolase, at 5,998,516 to 5,999,145 _5255
Group Condition PP_5251 PP_5252 PP_5253 PP_5254 PP_5255
temperature Growth at 25C -0.1 -0.1 -6.3 +0.6 +0.6
carbon source ferulate (C); with MOPS -0.3 -0.2 -2.1 -0.3 -0.3
nitrogen source nitrate (N) -0.1 -0.2 -2.6 -0.6 +0.5
pH Growth at pH8 and (C) D-Glucose +0.1 -0.0 -2.8 +0.2 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.1 -2.6 +0.1 -0.0
temperature shift Temperature shift 10_to_30; with MOPS -0.3 -0.1 -1.9 +0.3 -0.1
carbon source Tween 20 (C) -0.1 -0.3 -1.9 +0.1 +0.1
nitrogen source NAG (N); with MOPS -0.7 +0.3 -1.3 -0.1 +0.1
phage P. putida Sci small MOI 0.1 -0.3 -0.2 +0.2 -0.9 -0.7
carbon source L-Lysine 10 mM (C) +0.1 +0.1 -1.7 +0.1 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 +0.2 +0.2 -0.2 -1.8
nitrogen source 2,6-diaminopimelic (N) -0.6 -0.5 +0.1 +0.7 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.0 -0.3 -2.1 +0.4 +1.1
pH Growth at pH6 and (C) Trisodium citrate +0.1 -0.1 -1.5 +0.3 +0.4
stress Growth with Chloride 500 mM; with MOPS +0.1 +0.2 -1.5 +0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 +0.1 -0.9 +0.2 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 +0.1 -1.0 +0.3 +0.7
temperature shift Temperature shift 30_to_10; with MOPS -0.1 -0.0 +0.7 -0.8 +0.5
carbon source butyrate (C) +0.0 +0.3 -1.2 +0.5 +0.7
pH Growth at pH6 and (C) D-Glucose -0.5 -0.1 +0.2 -0.4 +1.2
carbon source carbon source 4-vinylphenol 0.1 mM -0.3 +0.2 -0.6 +0.7 +0.6
nitrogen source Propiolactam (N) +0.1 +0.2 +1.4 +0.2 -0.9
nitrogen source NAG (N); with MOPS +0.4 +0.3 +0.2 -1.1 +1.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.1 +1.3 +0.0 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.1 +1.2 -0.3 +0.5
pH Valerolactone (C) pH 6.5 -0.4 +0.2 +1.0 +0.2 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.3 -0.4 +0.5 +0.5
nitrogen source nitrate (N) -0.2 -0.1 +1.3 +0.4 +0.4
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.0 +0.4 +1.4 +0.5 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 +2.3 -0.3 +0.5
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