Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5249 and PP_5250 are separated by 203 nucleotidesPP_5250 and PP_5251 are separated by 1 nucleotidesPP_5251 and PP_5252 are separated by 176 nucleotidesPP_5252 and PP_5253 are separated by 199 nucleotides PP_5249: PP_5249 - Transcriptional regulator, LysR family, at 5,990,895 to 5,991,806 _5249 PP_5250: PP_5250 - proline-specific outer membrane porin D, at 5,992,010 to 5,993,272 _5250 PP_5251: PP_5251 - putative Transporter, at 5,993,274 to 5,994,872 _5251 PP_5252: PP_5252 - conserved exported protein of unknown function, at 5,995,049 to 5,997,031 _5252 PP_5253: PP_5253 - Arylesterase, at 5,997,231 to 5,998,049 _5253
Group Condition PP_5249 PP_5250 PP_5251 PP_5252 PP_5253
temperature Growth at 25C -0.2 +0.2 -0.1 -0.1 -6.3
pH Growth at pH8 and (C) D-Glucose -0.5 +0.3 +0.1 -0.0 -2.8
nitrogen source nitrate (N) -0.1 +0.1 -0.1 -0.2 -2.6
carbon source ferulate (C); with MOPS -0.2 -0.0 -0.3 -0.2 -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 +0.5 -0.0 -0.3 -2.1
carbon source Tween 20 (C) +0.1 -0.3 -0.1 -0.3 -1.9
temperature shift Temperature shift 10_to_30; with MOPS +0.2 -0.1 -0.3 -0.1 -1.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.2 +0.4 +0.1 -2.6
temperature Growth at 25C +0.2 +0.1 -0.5 -0.2 -1.1
carbon source L-Lysine 10 mM (C) +0.1 -0.1 +0.1 +0.1 -1.7
nitrogen source NAG (N); with MOPS +0.1 +0.3 -0.7 +0.3 -1.3
pH Growth at pH6 and (C) Trisodium citrate +0.1 +0.1 +0.1 -0.1 -1.5
stress Growth with Chloride 500 mM; with MOPS -0.1 +0.2 +0.1 +0.2 -1.5
temperature shift Temperature shift 30_to_25 -0.5 -0.1 -0.3 -0.5 +0.3
phage P. putida Sci Big MOI 0.1 -0.6 +0.1 +0.3 +0.0 -1.0
stress R2A with Fusaric 480 ug/mL +0.2 -0.0 +0.2 -0.0 -1.3
carbon source Valeric (C) +0.3 -0.3 +0.2 -0.1 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.4 +0.2 -0.2 +0.1 -1.2
nitrogen source D-2-Aminobutyric (N) -0.3 +0.0 -0.9 +0.2 +0.7
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.0 -0.5 -0.1 +1.3
carbon source carbon source 4-vinylphenol 0.1 mM +0.3 +0.5 -0.3 +0.2 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.2 +0.1 -0.5 +0.2 +0.1
nitrogen source nitrate (N) +0.3 -0.2 -0.2 -0.1 +1.3
temperature shift Temperature shift 10_to_30; with MOPS +0.3 +0.3 -0.3 +0.2 +0.8
stress R2A with Polymyxin B sulfate 0.001 mg/ml -0.6 +0.1 +0.0 +0.4 +1.4
pH Valerolactone (C) pH 6.5 +0.3 +0.3 -0.4 +0.2 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.2 -0.1 +0.1 +1.2
nitrogen source Propiolactam (N) +0.0 -0.1 +0.1 +0.2 +1.4
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.1 +0.3 +0.2 +0.3 +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.2 +0.2 +0.4 +2.3
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