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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5249 and PP_5250 are separated by 203 nucleotides
PP_5250 and PP_5251 are separated by 1 nucleotides
PP_5251 and PP_5252 are separated by 176 nucleotides
PP_5252 and PP_5253 are separated by 199 nucleotides
PP_5249: PP_5249 - Transcriptional regulator, LysR family, at 5,990,895 to 5,991,806
_5249
PP_5250: PP_5250 - proline-specific outer membrane porin D, at 5,992,010 to 5,993,272
_5250
PP_5251: PP_5251 - putative Transporter, at 5,993,274 to 5,994,872
_5251
PP_5252: PP_5252 - conserved exported protein of unknown function, at 5,995,049 to 5,997,031
_5252
PP_5253: PP_5253 - Arylesterase, at 5,997,231 to 5,998,049
_5253
Group
Condition
PP
_5249
PP
_5250
PP
_5251
PP
_5252
PP
_5253
temperature
Growth at 25C
-0.2
+0.2
-0.1
-0.1
-6.3
pH
Growth at pH8 and (C) D-Glucose
-0.5
+0.3
+0.1
-0.0
-2.8
nitrogen source
nitrate (N)
-0.1
+0.1
-0.1
-0.2
-2.6
carbon source
ferulate (C); with MOPS
-0.2
-0.0
-0.3
-0.2
-2.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.5
+0.5
-0.0
-0.3
-2.1
carbon source
Tween 20 (C)
+0.1
-0.3
-0.1
-0.3
-1.9
temperature shift
Temperature shift 10_to_30; with MOPS
+0.2
-0.1
-0.3
-0.1
-1.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.3
+0.2
+0.4
+0.1
-2.6
temperature
Growth at 25C
+0.2
+0.1
-0.5
-0.2
-1.1
carbon source
L-Lysine 10 mM (C)
+0.1
-0.1
+0.1
+0.1
-1.7
nitrogen source
NAG (N); with MOPS
+0.1
+0.3
-0.7
+0.3
-1.3
pH
Growth at pH6 and (C) Trisodium citrate
+0.1
+0.1
+0.1
-0.1
-1.5
stress
Growth with Chloride 500 mM; with MOPS
-0.1
+0.2
+0.1
+0.2
-1.5
temperature shift
Temperature shift 30_to_25
-0.5
-0.1
-0.3
-0.5
+0.3
phage
P. putida Sci Big MOI 0.1
-0.6
+0.1
+0.3
+0.0
-1.0
stress
R2A with Fusaric 480 ug/mL
+0.2
-0.0
+0.2
-0.0
-1.3
carbon source
Valeric (C)
+0.3
-0.3
+0.2
-0.1
-1.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.4
+0.2
-0.2
+0.1
-1.2
nitrogen source
D-2-Aminobutyric (N)
-0.3
+0.0
-0.9
+0.2
+0.7
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.4
-0.0
-0.5
-0.1
+1.3
carbon source
carbon source 4-vinylphenol 0.1 mM
+0.3
+0.5
-0.3
+0.2
-0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.2
+0.1
-0.5
+0.2
+0.1
nitrogen source
nitrate (N)
+0.3
-0.2
-0.2
-0.1
+1.3
temperature shift
Temperature shift 10_to_30; with MOPS
+0.3
+0.3
-0.3
+0.2
+0.8
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
-0.6
+0.1
+0.0
+0.4
+1.4
pH
Valerolactone (C) pH 6.5
+0.3
+0.3
-0.4
+0.2
+1.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
-0.2
-0.1
+0.1
+1.2
nitrogen source
Propiolactam (N)
+0.0
-0.1
+0.1
+0.2
+1.4
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
+0.1
+0.3
+0.2
+0.3
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
+0.2
+0.2
+0.4
+2.3
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