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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5244 and PP_5245 are separated by 138 nucleotides
PP_5245 and PP_5246 are separated by 198 nucleotides
PP_5246 and PP_5247 are separated by 583 nucleotides
PP_5247 and PP_5248 are separated by 348 nucleotides
PP_5244: PP_5244 - Transcriptional regulator, AraC family, at 5,985,191 to 5,985,595
_5244
PP_5245: PP_5245 - Transcriptional regulator, AraC family, at 5,985,734 to 5,986,720
_5245
PP_5246: PP_5246 - glutathione-regulated potassium/H+ antiporter, at 5,986,919 to 5,988,706
_5246
PP_5247: PP_5247 - conserved protein of unknown function, at 5,989,290 to 5,989,688
_5247
PP_5248: PP_5248 - putative hydrolase, at 5,990,037 to 5,990,666
_5248
Group
Condition
PP
_5244
PP
_5245
PP
_5246
PP
_5247
PP
_5248
stress
Growth with Chloride 500 mM; with MOPS
-0.1
-0.1
-0.2
+0.1
-3.7
pH
Growth at pH7 and (C) Trisodium citrate
+0.2
-0.3
+0.3
-0.4
-3.5
carbon source
carbon source 4-vinylphenol 0.01 mM
-0.4
-0.2
-0.5
-0.1
-2.3
carbon source
1,3-Butandiol (C)
-0.8
+0.2
+0.0
+0.5
-3.4
temperature shift
Temperature shift 10_to_25; with MOPS
+0.1
-0.1
-0.0
-1.7
-1.8
pH
Growth at pH6 and (C) Trisodium citrate
+0.0
-0.1
-0.0
+0.3
-3.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.1
-0.2
-0.2
-0.4
-2.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.3
-0.1
+0.3
+0.5
-3.4
temperature
Growth at 30C; with MOPS
-1.0
-0.1
+0.1
-0.5
-1.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
-0.1
+0.1
-0.5
-2.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.7
-0.2
+0.1
-0.2
-1.8
carbon source
1,4-Butanediol (C)
-0.5
-0.0
+0.0
-0.1
-2.2
no stress control
Growth with no stress
-0.1
-0.0
+0.2
+0.7
-3.5
carbon source
carbon source D-Glucose 55.5 mM
+0.1
-0.2
-0.4
-0.2
-2.1
pH
pH 4
-0.3
-0.1
-0.1
+0.1
-2.1
no phage control
Time6-nophage
-0.0
+0.3
-0.0
-1.2
-1.6
carbon source
ferulate (C); with MOPS
-0.2
+0.0
+0.3
-0.5
-2.0
carbon source
3-methyl-1-butanol 0.25 vol% (C)
+0.4
+0.1
-0.4
-0.4
-2.1
pH
Growth at pH6 and (C) D-Glucose
+0.3
-0.1
+0.0
+0.1
-2.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.3
-0.1
-0.2
-2.1
pH
Growth at pH6 and (C) Trisodium citrate
-1.1
-0.3
+0.0
+0.3
-0.9
carbon source
Sebacic (C)
+0.5
+0.1
-0.3
+0.1
-2.1
nitrogen source
nitrate (N)
+0.3
-0.0
+0.3
+0.4
-2.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.5
+0.2
+0.0
+0.2
-1.8
phage
P. putida PUT 7 MOI 1
-0.6
-0.4
-0.8
+0.2
+0.8
nitrogen source
NAG (N); with MOPS
+0.1
-0.4
+0.6
-1.6
+1.5
pH
Valerolactone (C) pH 6.5
-0.1
-0.1
+0.0
-0.5
+1.9
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.1
-0.2
-0.5
+1.5
-0.2
nitrogen source
nitrogen source nitrate 20mM
-0.2
+0.2
+0.3
+0.5
+1.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
+0.2
-0.1
+0.9
+4.7
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