Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5205 and PP_5206 are separated by 95 nucleotidesPP_5206 and PP_5207 overlap by 4 nucleotidesPP_5207 and PP_5208 are separated by 1 nucleotidesPP_5208 and PP_5722 are separated by 180 nucleotides PP_5205: PP_5205 - conserved protein of unknown function, at 5,935,939 to 5,936,388 _5205 PP_5206: PP_5206 - putative translation-related protein, at 5,936,484 to 5,937,443 _5206 PP_5207: PP_5207 - ribosome-associated ATPase, at 5,937,440 to 5,940,160 _5207 PP_5208: PP_5208 - putative translation related protein, at 5,940,162 to 5,941,274 _5208 PP_5722: PP_5722 - conserved protein of unknown function of phage origin, at 5,941,455 to 5,941,889 _5722
Group Condition PP_5205 PP_5206 PP_5207 PP_5208 PP_5722
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -1.1 -0.2 +0.0 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -1.0 +0.1 -0.0 -0.6 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.6 +0.2 -0.3 -0.3
carbon source L-Leucine (C) -0.4 -0.3 +0.1 -0.4 -0.2
nitrogen source Ammonium chloride (N) -0.6 -0.6 +0.1 -0.1 +0.2
pH pH 8 -0.9 -0.5 +0.0 +0.4 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 -0.2 -0.0 +0.3 -0.6
nitrogen source nitrate (N) +0.2 +0.1 -0.3 -0.4 -0.5
pH pH 5 -0.2 -1.1 -0.0 +0.5 +0.0
carbon source carbon source 4-vinylphenol 0.1 mM -0.6 -0.7 -0.1 +0.4 +0.1
nitrogen source NAG (N); with MOPS -0.4 -0.8 +0.2 +0.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 -0.2 +0.3 -0.4 -0.3
temperature shift Temperature shift 30_to_21; with MOPS +0.3 -0.7 -0.1 -0.4 +0.1
pH Growth at pH6 and (C) Trisodium citrate +0.1 -0.3 -0.5 -0.4 +0.3
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM -0.3 -0.5 -0.1 +0.4 -0.2
pH Growth at pH6 and (C) Trisodium citrate -0.3 -0.3 +0.2 -0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.4 -0.2 +0.4 +0.2
pH Growth at pH8 and (C) D-Glucose +0.5 +0.1 -0.3 -0.5 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 -0.0 +0.1 -0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.5 -0.0 +0.1 -0.5 -0.4
carbon source Tween 20 (C) +0.5 -0.5 +0.0 -0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.7 +0.1 -0.1 +0.3 +0.1
pH Growth at pH9 and (C) Trisodium citrate +0.4 -0.7 +0.1 +0.4 -0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM +0.2 -0.5 +0.3 +0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 -0.2 +0.1 -0.4 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.7 -0.3 +0.2 +0.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 -0.2 +0.1 +0.4 +0.6
stress R2A with Fusaric 480 ug/mL +0.3 +0.4 +0.4 +0.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 +0.6 +0.2 +0.4 +0.1
phage P. putida Sci small MOI 0.1 +0.0 +0.5 +0.3 +0.6 +0.4
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