Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5194 and PP_5195 are separated by 127 nucleotidesPP_5195 and PP_5196 are separated by 152 nucleotidesPP_5196 and PP_5197 are separated by 115 nucleotidesPP_5197 and PP_5198 are separated by 3 nucleotides PP_5194: PP_5194 - aminomethyltransferase, at 5,925,062 to 5,926,144 _5194 PP_5195: PP_5195 - putative Iron ABC transporter, permease protein, at 5,926,272 to 5,927,894 _5195 PP_5196: PP_5196 - putative iron(III)|iron ABC transporter, periplasmic iron-binding protein, at 5,928,047 to 5,929,048 _5196 PP_5197: PP_5197 - putative oxidoreductase involved in anerobic synthesis of ubiquinone, FAD/NAD(P)-binding domain, at 5,929,164 to 5,930,387 _5197 PP_5198: PP_5198 - conserved protein of unknown function, at 5,930,391 to 5,930,873 _5198
Group Condition PP_5194 PP_5195 PP_5196 PP_5197 PP_5198
carbon source acetate (C) -0.5 -0.0 +0.1 N.D. -3.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.3 +0.0 N.D. -3.3
pH Growth at pH6 and (C) D-Glucose -0.6 -0.3 +0.1 N.D. -2.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 +0.1 -0.0 N.D. -3.6
carbon source D-Glucose (C) -0.2 -0.1 +0.3 N.D. -2.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.5 -0.1 +0.2 N.D. -2.2
carbon source Delta-Undecalactone (C) -0.3 -0.0 -0.2 N.D. -2.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 -0.2 +0.2 N.D. -2.5
carbon source D-Mannose (C) +0.1 -0.2 +0.0 N.D. -2.2
carbon source Cholinium lysinate (C) -0.1 -0.1 -0.0 N.D. -2.1
nitrogen source nitrogen source nitrite 0.5mM -0.1 -0.1 +0.2 N.D. -2.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.1 +0.0 -0.1 N.D. -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.1 -0.0 +0.1 N.D. -2.0
nitrogen source NAG (N); with MOPS -0.1 -0.1 +0.2 N.D. -2.1
pH Growth at pH6 and (C) D-Glucose +0.2 -0.1 +0.3 N.D. -2.1
temperature Growth at 30C; with MOPS +0.2 -0.1 +0.1 N.D. -1.7
carbon source Tween 20 (C) +0.4 -0.3 +0.1 N.D. -1.7
carbon source 4-Hydroxyvalerate (C) (40mM) +0.6 -0.2 +0.0 N.D. -1.8
nitrogen source no extra (N); with MOPS +0.3 -0.0 +0.2 N.D. -1.8
carbon source Oleic (C) +0.3 +0.1 -0.3 N.D. -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 +0.2 +0.2 N.D. -1.7
temperature shift Temperature shift 10_to_25; with MOPS -0.2 +0.3 +0.4 N.D. -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 -0.1 +0.2 N.D. -1.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.1 +0.1 N.D. -1.6
carbon source Hexanoic (C) +0.7 -0.2 -0.1 N.D. -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.4 +0.3 +0.2 N.D. -1.6
carbon source ferulate (C); with MOPS +0.4 -0.1 +0.4 N.D. -1.4
carbon source 3-methyl-1-butanol (C) +0.2 +0.9 +1.1 N.D. -0.4
pH Growth at pH9 and (C) Trisodium citrate +0.3 -0.2 +0.4 N.D. +1.5
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.0 +0.7 +0.7 N.D. +2.1
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