Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5189 and PP_5190 are separated by 107 nucleotidesPP_5190 and PP_5191 are separated by 88 nucleotidesPP_5191 and PP_5192 are separated by 65 nucleotidesPP_5192 and PP_5193 are separated by 196 nucleotides PP_5189: PP_5189 - conserved protein of unknown function, at 5,919,002 to 5,919,307 _5189 PP_5190: PP_5190 - Type II secretion system protein, at 5,919,415 to 5,921,088 _5190 PP_5191: PP_5191 - conserved exported protein of unknown function, at 5,921,177 to 5,921,491 _5191 PP_5192: PP_5192 - glycine dehydrogenase, at 5,921,557 to 5,924,430 _5192 PP_5193: PP_5193 - glycine cleavage system H protein 2, at 5,924,627 to 5,925,010 _5193
Group Condition PP_5189 PP_5190 PP_5191 PP_5192 PP_5193
nitrogen source D-2-Aminobutyric (N) N.D. +0.4 -3.5 +0.2 +0.0
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.2 -1.7 -0.0 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -0.2 -1.4 -0.1 -0.2
pH D-Glucose (C) pH 6 N.D. -0.2 -1.5 +0.1 -0.2
carbon source Trisodium citrate (C) N.D. +0.3 -1.7 +0.2 -0.5
carbon source L-Alanine (C) N.D. -0.3 -0.7 +0.1 -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.0 -1.3 +0.1 -0.2
nitrogen source no extra (N); with MOPS N.D. -0.1 -1.4 +0.2 +0.0
nitrogen source 2,6-diaminopimelic (N) N.D. +0.3 -1.6 +0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 -1.0 +0.2 -0.5
phage P. put South MOI 10 N.D. +0.3 -1.5 -0.0 -0.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. -0.5 -0.9 -0.1 +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.6 -0.1 +0.4 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.3 -1.3 -0.1 +0.2
carbon source D-Lysine 10 mM (C) N.D. +0.1 -1.4 +0.2 +0.2
carbon source acetate (C) N.D. -0.6 -0.6 -0.1 +0.5
phage P. putida Sci small MOI 0.1 N.D. -0.6 +0.5 -0.2 -0.4
nitrogen source Urea (N) N.D. -0.4 +0.9 +0.0 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.4 -1.0 +0.5 -0.1
pH Growth at pH6 and (C) D-Glucose N.D. -0.4 +0.7 -0.4 +0.3
carbon source carbon source 2-Deoxy-D-glucose 10 mM N.D. -0.4 +1.0 -0.1 -0.2
temperature shift Temperature shift 30_to_21; with MOPS N.D. +0.2 +0.8 +0.2 -0.5
carbon source ferulate (C); with MOPS N.D. -0.2 +1.2 -0.4 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -0.5 +0.8 +0.2 +0.4
nitrogen source nitrogen source nitrite 1mM N.D. -0.4 +1.2 +0.0 +0.1
pH pH 7 N.D. +0.3 +1.5 +0.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. +0.7 +0.7 +0.2 -0.1
pH pH 6 N.D. +0.4 +1.2 -0.1 +0.0
pH Growth at pH6 and (C) Trisodium citrate N.D. +0.5 +0.6 +0.2 +0.5
stress Growth with Chloride 200 mM N.D. +0.1 +1.4 +0.1 +0.1
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