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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5135 and PP_5136 are separated by 18 nucleotides
PP_5136 and PP_5137 are separated by 1 nucleotides
PP_5137 and PP_5138 are separated by 230 nucleotides
PP_5138 and PP_5139 are separated by 9 nucleotides
PP_5135: PP_5135 - ABC transporter, periplasmic binding protein, at 5,858,960 to 5,859,985
_5135
PP_5136: PP_5136 - ABC transporter, permease protein, at 5,860,004 to 5,861,728
_5136
PP_5137: PP_5137 - ferric iron ABC transporter, ATP-binding protein, at 5,861,730 to 5,862,788
_5137
PP_5138: PP_5138 - putative transcriptional regulator LysR family, at 5,863,019 to 5,863,888
_5138
PP_5139: PP_5139 - cadmium translocating P-type ATPase, at 5,863,898 to 5,866,150
_5139
Group
Condition
PP
_5135
PP
_5136
PP
_5137
PP
_5138
PP
_5139
micoeukaryotes
C.elegans, mixed culture-1
-0.1
-0.0
-0.2
+0.0
-3.6
carbon source
3-methyl-1-butanol 0.25 vol% (C)
+0.1
+0.0
-0.7
+0.2
-2.9
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.0
+0.1
+0.0
+0.3
-3.8
nitrogen source
NAG (N); with MOPS
-0.4
-0.0
+0.0
+0.0
-2.8
carbon source
3-methyl-1-butanol 0.1 vol% (C)
-0.1
+0.1
+0.2
+0.2
-3.3
micoeukaryotes
Tetrahymena sp., Mixed culture-2
+0.5
-0.1
-0.2
+0.2
-3.1
phage
P. putida Sci small MOI 0.1
-0.7
-0.2
-0.7
+0.0
-0.6
micoeukaryotes
Acanthamoeba sp., muxed culture-2
-0.0
+0.3
-0.2
+0.3
-2.4
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.1
-0.1
-0.8
-0.1
-0.8
supernatant
LB 0.1x with 55.5 mM D-Glucose with Supernatant: Acinetobacter
+0.0
+0.1
-0.3
-0.1
-1.7
stress
R2A with Fusaric 240 ug/mL
-0.2
+0.2
-0.2
-0.0
-1.5
pH
Growth at pH6 and (C) Trisodium citrate
-0.1
+0.1
-1.1
-0.3
-0.4
pH
Growth at pH6 and (C) Trisodium citrate
-0.2
-0.3
+0.1
+0.3
-1.6
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
+0.1
-0.8
+0.1
-1.1
dilutelb
LB 0.1x with 55.5 mM D-Glucose
-0.2
-0.0
+0.2
+0.0
-1.6
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
+0.0
-0.0
+0.1
+0.1
-1.7
carbon source
acetate (C)
+0.1
-0.0
-0.4
+0.2
-1.3
temperature shift
Temperature shift 30_to_25
-0.5
+0.1
+0.2
+0.1
-1.4
temperature
Growth at 25C
+0.1
+0.0
-0.7
+0.5
-1.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.3
-0.2
+0.1
-0.1
-1.4
nitrogen source
no extra (N); with MOPS
-0.4
-0.1
-0.3
+0.3
-0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
+0.2
+0.1
+0.2
-0.9
nitrogen source
nitrogen source nitrite 0.5mM
+0.2
+0.2
-0.5
+0.1
-1.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.4
+0.0
+0.4
+0.2
-1.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.3
+0.2
+0.4
-0.2
-1.1
phage
P. putida Sci Big MOI 0.1
-0.7
-0.2
-0.3
-0.3
+0.5
nitrogen source
D-2-Aminobutyric (N)
-0.2
+0.2
+0.6
-0.8
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.2
+0.3
+0.3
+0.5
-0.8
pH
pH 8
-0.5
-0.1
+0.6
+0.0
+0.9
carbon source
carbon source 3-O-methyl-glucose 50 mM
+0.2
-0.1
+0.8
-0.1
+0.6
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