Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5135 and PP_5136 are separated by 18 nucleotidesPP_5136 and PP_5137 are separated by 1 nucleotidesPP_5137 and PP_5138 are separated by 230 nucleotidesPP_5138 and PP_5139 are separated by 9 nucleotides PP_5135: PP_5135 - ABC transporter, periplasmic binding protein, at 5,858,960 to 5,859,985 _5135 PP_5136: PP_5136 - ABC transporter, permease protein, at 5,860,004 to 5,861,728 _5136 PP_5137: PP_5137 - ferric iron ABC transporter, ATP-binding protein, at 5,861,730 to 5,862,788 _5137 PP_5138: PP_5138 - putative transcriptional regulator LysR family, at 5,863,019 to 5,863,888 _5138 PP_5139: PP_5139 - cadmium translocating P-type ATPase, at 5,863,898 to 5,866,150 _5139
Group Condition PP_5135 PP_5136 PP_5137 PP_5138 PP_5139
micoeukaryotes C.elegans, mixed culture-1 -0.1 -0.0 -0.2 +0.0 -3.6
carbon source 3-methyl-1-butanol 0.25 vol% (C) +0.1 +0.0 -0.7 +0.2 -2.9
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.0 +0.1 +0.0 +0.3 -3.8
nitrogen source NAG (N); with MOPS -0.4 -0.0 +0.0 +0.0 -2.8
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.1 +0.1 +0.2 +0.2 -3.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.5 -0.1 -0.2 +0.2 -3.1
phage P. putida Sci small MOI 0.1 -0.7 -0.2 -0.7 +0.0 -0.6
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.0 +0.3 -0.2 +0.3 -2.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.1 -0.1 -0.8 -0.1 -0.8
supernatant LB 0.1x with 55.5 mM D-Glucose with Supernatant: Acinetobacter +0.0 +0.1 -0.3 -0.1 -1.7
stress R2A with Fusaric 240 ug/mL -0.2 +0.2 -0.2 -0.0 -1.5
pH Growth at pH6 and (C) Trisodium citrate -0.1 +0.1 -1.1 -0.3 -0.4
pH Growth at pH6 and (C) Trisodium citrate -0.2 -0.3 +0.1 +0.3 -1.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.1 -0.8 +0.1 -1.1
dilutelb LB 0.1x with 55.5 mM D-Glucose -0.2 -0.0 +0.2 +0.0 -1.6
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.0 -0.0 +0.1 +0.1 -1.7
carbon source acetate (C) +0.1 -0.0 -0.4 +0.2 -1.3
temperature shift Temperature shift 30_to_25 -0.5 +0.1 +0.2 +0.1 -1.4
temperature Growth at 25C +0.1 +0.0 -0.7 +0.5 -1.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.2 +0.1 -0.1 -1.4
nitrogen source no extra (N); with MOPS -0.4 -0.1 -0.3 +0.3 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.2 +0.1 +0.2 -0.9
nitrogen source nitrogen source nitrite 0.5mM +0.2 +0.2 -0.5 +0.1 -1.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.0 +0.4 +0.2 -1.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 +0.2 +0.4 -0.2 -1.1
phage P. putida Sci Big MOI 0.1 -0.7 -0.2 -0.3 -0.3 +0.5
nitrogen source D-2-Aminobutyric (N) -0.2 +0.2 +0.6 -0.8 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 +0.3 +0.3 +0.5 -0.8
pH pH 8 -0.5 -0.1 +0.6 +0.0 +0.9
carbon source carbon source 3-O-methyl-glucose 50 mM +0.2 -0.1 +0.8 -0.1 +0.6
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