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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5078 and PP_5079 are separated by 96 nucleotides
PP_5079 and PP_5080 are separated by 4 nucleotides
PP_5080 and PP_5081 overlap by 53 nucleotides
PP_5081 and PP_5082 overlap by 4 nucleotides
PP_5078: PP_5078 - 3-dehydroquinate synthase, at 5,799,878 to 5,800,975
_5078
PP_5079: PP_5079 - Shikimate kinase, at 5,801,072 to 5,801,590
_5079
PP_5080: PP_5080 - type 4 pili biogenesis protein, at 5,801,595 to 5,802,833
_5080
PP_5081: PP_5081 - Type IV pili biogenesis protein, at 5,802,781 to 5,803,797
_5081
PP_5082: PP_5082 - Type IV pili biogenesis protein PilN, at 5,803,794 to 5,804,330
_5082
Group
Condition
PP
_5078
PP
_5079
PP
_5080
PP
_5081
PP
_5082
carbon source
Delta-Nonalactone (C)
N.D.
N.D.
-0.6
-0.5
-0.3
pH
pH 8
N.D.
N.D.
-1.1
-0.2
+0.0
phage
P. putida PUT 7 MOI 1
N.D.
N.D.
-0.9
-0.6
+0.2
phage
P. putida Sci Big MOI 0.1
N.D.
N.D.
-0.2
-0.3
-0.6
carbon source
Tween 20 (C)
N.D.
N.D.
-0.1
-0.4
-0.6
nitrogen source
2,6-diaminopimelic (N)
N.D.
N.D.
+0.0
-0.2
-0.9
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM
N.D.
N.D.
-0.7
-0.0
-0.3
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM
N.D.
N.D.
-1.0
+0.1
-0.1
carbon source
Delta-Decalactone (C)
N.D.
N.D.
-0.7
-0.3
+0.0
carbon source
acetate (C)
N.D.
N.D.
-1.0
+0.1
+0.0
nitrogen source
D-2-Aminobutyric (N)
N.D.
N.D.
-0.6
-0.0
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.0
+0.0
-0.9
pH
Growth at pH7 and (C) D-Glucose
N.D.
N.D.
-0.9
+0.2
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
N.D.
-0.5
+0.1
-0.4
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
N.D.
N.D.
-0.8
+0.2
+0.2
carbon source
3-methyl-1-butanol 0.1 vol% (C)
N.D.
N.D.
-0.6
-0.1
+0.4
temperature shift
Temperature shift 10_to_34; with MOPS
N.D.
N.D.
-0.6
+0.1
+0.2
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
N.D.
N.D.
-0.4
-0.2
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
N.D.
N.D.
-0.6
+0.2
+0.3
pH
Growth at pH6 and (C) Trisodium citrate
N.D.
N.D.
-0.6
+0.3
+0.2
temperature shift
Temperature shift 10_to_25; with MOPS
N.D.
N.D.
-0.4
+0.3
+0.4
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
N.D.
N.D.
-0.3
+0.2
+0.4
carbon source
ferulate (C); with MOPS
N.D.
N.D.
+0.7
+0.1
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.3
-0.1
+0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
N.D.
+0.8
+0.1
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.3
+0.5
+0.1
temperature
Growth at 25C
N.D.
N.D.
+0.4
+0.2
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.6
+0.1
+0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.8
+0.2
+0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.8
+0.1
+0.8
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