Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5078 and PP_5079 are separated by 96 nucleotidesPP_5079 and PP_5080 are separated by 4 nucleotidesPP_5080 and PP_5081 overlap by 53 nucleotidesPP_5081 and PP_5082 overlap by 4 nucleotides PP_5078: PP_5078 - 3-dehydroquinate synthase, at 5,799,878 to 5,800,975 _5078 PP_5079: PP_5079 - Shikimate kinase, at 5,801,072 to 5,801,590 _5079 PP_5080: PP_5080 - type 4 pili biogenesis protein, at 5,801,595 to 5,802,833 _5080 PP_5081: PP_5081 - Type IV pili biogenesis protein, at 5,802,781 to 5,803,797 _5081 PP_5082: PP_5082 - Type IV pili biogenesis protein PilN, at 5,803,794 to 5,804,330 _5082
Group Condition PP_5078 PP_5079 PP_5080 PP_5081 PP_5082
carbon source Delta-Nonalactone (C) N.D. N.D. -0.6 -0.5 -0.3
pH pH 8 N.D. N.D. -1.1 -0.2 +0.0
phage P. putida PUT 7 MOI 1 N.D. N.D. -0.9 -0.6 +0.2
phage P. putida Sci Big MOI 0.1 N.D. N.D. -0.2 -0.3 -0.6
carbon source Tween 20 (C) N.D. N.D. -0.1 -0.4 -0.6
nitrogen source 2,6-diaminopimelic (N) N.D. N.D. +0.0 -0.2 -0.9
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM N.D. N.D. -0.7 -0.0 -0.3
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM N.D. N.D. -1.0 +0.1 -0.1
carbon source Delta-Decalactone (C) N.D. N.D. -0.7 -0.3 +0.0
carbon source acetate (C) N.D. N.D. -1.0 +0.1 +0.0
nitrogen source D-2-Aminobutyric (N) N.D. N.D. -0.6 -0.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +0.0 +0.0 -0.9
pH Growth at pH7 and (C) D-Glucose N.D. N.D. -0.9 +0.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.5 +0.1 -0.4
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM N.D. N.D. -0.8 +0.2 +0.2
carbon source 3-methyl-1-butanol 0.1 vol% (C) N.D. N.D. -0.6 -0.1 +0.4
temperature shift Temperature shift 10_to_34; with MOPS N.D. N.D. -0.6 +0.1 +0.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. N.D. -0.4 -0.2 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. N.D. -0.6 +0.2 +0.3
pH Growth at pH6 and (C) Trisodium citrate N.D. N.D. -0.6 +0.3 +0.2
temperature shift Temperature shift 10_to_25; with MOPS N.D. N.D. -0.4 +0.3 +0.4
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. N.D. -0.3 +0.2 +0.4
carbon source ferulate (C); with MOPS N.D. N.D. +0.7 +0.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.3 -0.1 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. N.D. +0.8 +0.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.3 +0.5 +0.1
temperature Growth at 25C N.D. N.D. +0.4 +0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.6 +0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.8 +0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.8 +0.1 +0.8
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