Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5077 and PP_5078 are separated by 15 nucleotidesPP_5078 and PP_5079 are separated by 96 nucleotidesPP_5079 and PP_5080 are separated by 4 nucleotidesPP_5080 and PP_5081 overlap by 53 nucleotides PP_5077: PP_5077 - conserved protein of unknown function, at 5,798,273 to 5,799,862 _5077 PP_5078: PP_5078 - 3-dehydroquinate synthase, at 5,799,878 to 5,800,975 _5078 PP_5079: PP_5079 - Shikimate kinase, at 5,801,072 to 5,801,590 _5079 PP_5080: PP_5080 - type 4 pili biogenesis protein, at 5,801,595 to 5,802,833 _5080 PP_5081: PP_5081 - Type IV pili biogenesis protein, at 5,802,781 to 5,803,797 _5081
Group Condition PP_5077 PP_5078 PP_5079 PP_5080 PP_5081
dilutelb LB 0.1x with 55.5 mM D-Glucose -3.5 N.D. N.D. +0.1 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 -1.7 N.D. N.D. -0.5 -0.1
pH pH 8 -0.8 N.D. N.D. -1.1 -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 -2.2 N.D. N.D. +0.2 -0.1
supernatant LB 0.1x with 55.5 mM D-Glucose with Supernatant: Acinetobacter -2.7 N.D. N.D. +0.3 +0.4
phage P. putida PUT 7 MOI 1 -0.1 N.D. N.D. -0.9 -0.6
phage P. putida Sci small MOI 0.1 -1.0 N.D. N.D. -0.3 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.5 N.D. N.D. -0.4 -0.3
dilutelb LB 0.1x with 55.5 mM D-Glucose -1.5 N.D. N.D. +0.2 +0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -0.1 N.D. N.D. -1.0 +0.1
micoeukaryotes C.elegans, mixed culture-1 -1.1 N.D. N.D. +0.1 -0.0
micoeukaryotes Acanthamoeba sp., muxed culture-1 -1.0 N.D. N.D. +0.1 +0.1
carbon source carbon source Bisabolol 45 mM -0.8 N.D. N.D. +0.2 -0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -0.2 N.D. N.D. -0.8 +0.2
pH pH 9 -1.0 N.D. N.D. +0.4 -0.2
carbon source L-tyrosine 5 mM (C) -0.8 N.D. N.D. -0.2 +0.3
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB -0.9 N.D. N.D. +0.1 +0.1
pH pH 5 -0.9 N.D. N.D. +0.2 +0.0
pH Growth at pH7 and (C) D-Glucose +0.1 N.D. N.D. -0.9 +0.2
carbon source acetate (C) +0.3 N.D. N.D. -1.0 +0.1
carbon source 2-Methylbutanoic (C) -1.0 N.D. N.D. +0.1 +0.3
carbon source carbon source 3-O-methyl-glucose 10 mM -0.6 N.D. N.D. +0.3 -0.2
carbon source ferulate (C); with MOPS -0.9 N.D. N.D. +0.4 -0.0
carbon source Delta-Nonalactone (C) +0.8 N.D. N.D. -0.6 -0.5
carbon source Delta-Decalactone (C) +0.9 N.D. N.D. -0.7 -0.3
nitrogen source D-2-Aminobutyric (N) +0.7 N.D. N.D. -0.6 -0.0
nitrogen source D-2-Aminobutyric (N) +0.7 N.D. N.D. -0.2 +0.3
temperature Growth at 25C +0.5 N.D. N.D. +0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. N.D. +0.8 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 N.D. N.D. +0.8 +0.1
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