Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4987 and PP_4988 overlap by 8 nucleotidesPP_4988 and PP_4989 are separated by 12 nucleotidesPP_4989 and PP_4990 are separated by 11 nucleotidesPP_4990 and PP_4991 overlap by 4 nucleotides PP_4987: PP_4987 - putative Chemotaxis protein, at 5,681,486 to 5,681,959 _4987 PP_4988: PP_4988 - signal transduction histidine kinase CheA/CheY-like receiver, at 5,681,952 to 5,686,892 _4988 PP_4989: PP_4989 - Protein PilJ, at 5,686,905 to 5,688,965 _4989 PP_4990: PP_4990 - Protein PilI, at 5,688,977 to 5,689,531 _4990 PP_4991: PP_4991 - Protein PilH, at 5,689,528 to 5,689,893 _4991
Group Condition PP_4987 PP_4988 PP_4989 PP_4990 PP_4991
pH Growth at pH7 and (C) D-Glucose N.D. -1.7 -1.7 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -2.3 -0.9 N.D. N.D.
nitrogen source NAG (N); with MOPS N.D. -1.7 -1.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.1 -1.9 N.D. N.D.
nitrogen source nitrogen source nitrite 0.5mM N.D. -1.5 -1.3 N.D. N.D.
carbon source D-Glucose (C) N.D. -1.5 -1.2 N.D. N.D.
carbon source Tween 20 (C) N.D. -1.6 -1.1 N.D. N.D.
phage P. putida Sci Big MOI 0.1 N.D. -0.2 -2.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.7 -1.0 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -1.2 -1.4 N.D. N.D.
carbon source butyrate (C) N.D. -0.4 -2.2 N.D. N.D.
pH Growth at pH7 and (C) D-Glucose N.D. -0.6 -2.0 N.D. N.D.
carbon source Acetate Carbon Source (5mM) N.D. -1.3 -1.3 N.D. N.D.
phage P. putida Sci small MOI 0.1 N.D. -0.6 -2.0 N.D. N.D.
temperature LB_noSalt 37C N.D. -1.0 -1.6 N.D. N.D.
stress R2A with Fusaric 240 ug/mL N.D. -0.9 -1.6 N.D. N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.0 -1.4 N.D. N.D.
nitrogen source Ammonium chloride (N) N.D. -0.9 -1.5 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -1.9 +0.5 N.D. N.D.
temperature shift Temperature shift 30_to_4 N.D. -2.0 +0.7 N.D. N.D.
temperature shift Temperature shift 30_to_4; with MOPS N.D. -1.8 +0.7 N.D. N.D.
temperature shift Temperature shift 30_to_4; with MOPS N.D. -1.5 +1.2 N.D. N.D.
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. N.D. +1.3 +1.1 N.D. N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay N.D. +1.4 +1.2 N.D. N.D.
phage P. putida South MOI 0.1 N.D. +1.1 +1.5 N.D. N.D.
phage P. put South MOI 1 N.D. +1.2 +1.5 N.D. N.D.
phage P. putida PUT10 MOI 10 N.D. +1.2 +1.6 N.D. N.D.
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. N.D. +1.7 +1.2 N.D. N.D.
carbon source DL-2-Aminoadipic 10 mM (C) N.D. +1.4 +1.6 N.D. N.D.
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. N.D. +2.1 +1.8 N.D. N.D.
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