Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4966 and PP_4967 are separated by 18 nucleotidesPP_4967 and PP_4968 are separated by 228 nucleotidesPP_4968 and PP_4969 are separated by 132 nucleotidesPP_4969 and PP_4970 are separated by 74 nucleotides PP_4966: PP_4966 - transcriptional regulator of methionine metabolism, at 5,657,946 to 5,658,938 _4966 PP_4967: PP_4967 - methionine adenosyltransferase, at 5,658,957 to 5,660,147 _4967 PP_4968: PP_4968 - DNA ligase B, at 5,660,376 to 5,662,076 _4968 PP_4969: PP_4969 - conserved exported protein of unknown function, at 5,662,209 to 5,662,601 _4969 PP_4970: PP_4970 - putative Cytochrome c', at 5,662,676 to 5,663,143 _4970
Group Condition PP_4966 PP_4967 PP_4968 PP_4969 PP_4970
pH Growth at pH7 and (C) Trisodium citrate -6.7 N.D. +0.1 N.D. N.D.
stress Growth with Chloride 200 mM -2.9 N.D. +0.1 N.D. N.D.
pH Valerolactone (C) pH 6.5 -2.1 N.D. -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.8 N.D. -0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.9 N.D. -0.1 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -2.5 N.D. +0.4 N.D. N.D.
carbon source L-tyrosine 5 mM (C) -1.6 N.D. -0.4 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -2.1 N.D. +0.2 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -2.0 N.D. +0.2 N.D. N.D.
carbon source D-Glucose (C); with MOPS -2.0 N.D. +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 N.D. -0.2 N.D. N.D.
nitrogen source nitrogen source nitrite 0.5mM -1.5 N.D. -0.1 N.D. N.D.
pH Growth at pH6 and (C) D-Glucose -1.4 N.D. -0.1 N.D. N.D.
temperature shift Temperature shift 10_to_25; with MOPS -1.6 N.D. +0.2 N.D. N.D.
nitrogen source Propiolactam (N) -1.1 N.D. -0.4 N.D. N.D.
stress MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL -1.4 N.D. -0.1 N.D. N.D.
carbon source carbon source 3-O-methyl-glucose 50 mM -1.4 N.D. +0.0 N.D. N.D.
temperature Growth at 25C -1.2 N.D. -0.1 N.D. N.D.
pH D-Glucose (C) pH 7.5 -1.3 N.D. +0.1 N.D. N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Phenylalanine 5 mM -1.4 N.D. +0.2 N.D. N.D.
carbon source 3-methyl-3-buten-1-ol 0.25 vol% (C) -1.3 N.D. +0.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 N.D. +0.2 N.D. N.D.
temperature Growth at 25C; with MOPS -1.2 N.D. +0.2 N.D. N.D.
micoeukaryotes Dictyostellum sp.mixed culture-2 -1.3 N.D. +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.2 N.D. +0.2 N.D. N.D.
carbon source ferulate (C); with MOPS -1.3 N.D. +0.5 N.D. N.D.
pH pH 4 -0.9 N.D. +0.4 N.D. N.D.
nitrogen source 2,6-diaminopimelic (N) +1.1 N.D. -0.6 N.D. N.D.
carbon source ferulate (C); with MOPS +1.1 N.D. -0.3 N.D. N.D.
pH Growth at pH6 and (C) Trisodium citrate +1.4 N.D. -0.2 N.D. N.D.
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