Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4962 and PP_4963 overlap by 1 nucleotidesPP_4963 and PP_4964 are separated by 12 nucleotidesPP_4964 and PP_4965 are separated by 151 nucleotidesPP_4965 and PP_4966 are separated by 251 nucleotides PP_4962: PP_4962 - conserved exported protein of unknown function, at 5,653,060 to 5,653,311 _4962 PP_4963: PP_4963 - Phosphoglycerate kinase, at 5,653,311 to 5,654,474 _4963 PP_4964: PP_4964 - D-erythrose 4-phosphate dehydrogenase, at 5,654,487 to 5,655,545 _4964 PP_4965: PP_4965 - transketolase A, at 5,655,697 to 5,657,694 _4965 PP_4966: PP_4966 - transcriptional regulator of methionine metabolism, at 5,657,946 to 5,658,938 _4966
Group Condition PP_4962 PP_4963 PP_4964 PP_4965 PP_4966
pH Growth at pH7 and (C) Trisodium citrate N.D. N.D. -2.2 N.D. -6.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. N.D. -2.9 N.D. -2.0
stress Growth with Chloride 200 mM N.D. N.D. -1.5 N.D. -2.9
nitrogen source NAG (N); with MOPS N.D. N.D. -3.5 N.D. -0.6
no stress control Growth with no stress N.D. N.D. -3.0 N.D. -1.0
micoeukaryotes Dictyostellum sp.mixed culture-2 N.D. N.D. -2.7 N.D. -1.3
phage P. putida South MOI 10 N.D. N.D. -2.8 N.D. -1.0
pH Growth at pH6 and (C) D-Glucose N.D. N.D. -2.2 N.D. -1.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. N.D. -3.8 N.D. +0.1
carbon source ferulate (C); with MOPS N.D. N.D. -2.3 N.D. -1.3
temperature Growth at 25C N.D. N.D. -2.2 N.D. -1.2
carbon source Vanillin (C) N.D. N.D. -3.4 N.D. +0.0
temperature shift Temperature shift 10_to_34; with MOPS N.D. N.D. -3.3 N.D. -0.1
carbon source Levulinic Acid (C) N.D. N.D. -3.1 N.D. -0.2
carbon source 3-hydroxypentanoate N.D. N.D. -2.3 N.D. -1.0
pH Growth at pH6 and (C) Trisodium citrate N.D. N.D. -3.2 N.D. -0.1
pH Growth at pH9 and (C) Trisodium citrate N.D. N.D. -2.2 N.D. -1.1
stress R2A with Fusaric 240 ug/mL N.D. N.D. -2.6 N.D. -0.7
carbon source D-Glucose (C) N.D. N.D. -3.4 N.D. +0.1
pH Growth at pH9 and (C) Trisodium citrate N.D. N.D. -2.9 N.D. +0.4
carbon source 3-methyl-1-butanol (C) N.D. N.D. -3.0 N.D. +0.6
no stress control Growth with no stress N.D. N.D. -3.1 N.D. +0.8
pH Growth at pH6 and (C) Trisodium citrate N.D. N.D. -3.1 N.D. +0.9
pH Growth at pH6 and (C) Trisodium citrate N.D. N.D. -3.4 N.D. +1.4
carbon source ferulate (C); with MOPS N.D. N.D. -2.4 N.D. +1.1
pH Valerolactone (C) pH 6.5 N.D. N.D. +1.6 N.D. -2.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +2.3 N.D. -1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +2.5 N.D. -1.0
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. N.D. +2.6 N.D. -0.7
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. N.D. N.D. +3.2 N.D. -0.1
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