Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4951 and PP_4952 are separated by 64 nucleotidesPP_4952 and PP_4953 are separated by 122 nucleotidesPP_4953 and PP_4954 are separated by 128 nucleotidesPP_4954 and PP_4955 are separated by 18 nucleotides PP_4951: PP_4951 - Drug resistance transporter, EmrB/QacA family, at 5,640,338 to 5,641,765 _4951 PP_4952: PP_4952 - ATP-dependent RNA helicase, specific for 23S rRNA, at 5,641,830 to 5,643,215 _4952 PP_4953: PP_4953 - putative oxidoreductase with FAD/NAD(P)-binding domain, at 5,643,338 to 5,644,519 _4953 PP_4954: PP_4954 - conserved membrane protein of unknown function, at 5,644,648 to 5,646,831 _4954 PP_4955: PP_4955 - conserved exported protein of unknown function, at 5,646,850 to 5,647,587 _4955
Group Condition PP_4951 PP_4952 PP_4953 PP_4954 PP_4955
phage PUT8 MOI 0.1 +0.2 -5.1 +0.3 -0.5 -0.0
nitrogen source nitrate (N) +0.4 -4.1 -0.3 -0.1 +0.1
nitrogen source NAG (N); with MOPS +0.0 -4.1 -0.5 +0.4 +0.2
carbon source Trisodium citrate (C) -0.0 -4.2 +0.2 +0.3 +0.3
nitrogen source D-2-Aminobutyric (N) -0.3 -2.8 -0.1 -0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -2.6 -0.2 +0.1 +0.0
carbon source 2-Keto-D-gluconic hemicalcium salt (C) +0.2 -3.2 -0.1 +0.0 +0.1
pH Growth at pH6 and (C) D-Glucose +0.4 -4.2 +0.4 +0.3 +0.2
nitrogen source 2,6-diaminopimelic (N) -0.4 -2.3 -0.4 -0.1 +0.4
phage P. putida PUT10 MOI 10 -0.2 -2.1 -0.0 -0.4 +0.1
carbon source L-Leucine (C) +0.1 -2.3 -0.4 -0.1 +0.0
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM -1.6 -0.6 +0.0 -0.5 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -2.5 -0.5 -0.0 +0.3
carbon source Glutaric (C) +0.1 -2.2 +0.1 -0.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.0 -2.7 -0.0 +0.7 +0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 -3.0 +0.5 +0.4 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 -2.8 +0.5 +0.3 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -0.6 -1.6 +0.5 +0.3
temperature shift Temperature shift 30_to_10; with MOPS +0.1 -2.0 +0.4 +0.1 +0.2
nitrogen source Ammonium chloride (N) +0.1 -2.3 +0.4 +0.5 +0.2
carbon source Valerolactone (C) +0.1 -2.2 +0.7 -0.0 +0.3
pH Growth at pH7 and (C) D-Glucose -0.0 -1.9 +0.5 +0.3 +0.2
pH Growth at pH6 and (C) Trisodium citrate -0.5 -1.1 +0.9 +0.1 +0.4
carbon source D-Glucose (C) +0.3 +0.6 -1.8 +1.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.7 -1.2 +0.5 +0.3 +0.3
phage P. putida Sci small MOI 0.1 -0.4 +1.6 +0.7 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +1.9 -0.3 +0.2 +0.6
temperature Growth at 25C +0.4 +1.0 +0.5 +0.8 +0.3
nitrogen source NAG (N); with MOPS +0.6 +1.3 +0.3 +0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.7 +1.7 -0.9 +0.2 +0.5
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